Method for stabilizing cholesterol oxidase
The present invention provides a method for stabilizing a cholesterol oxidase, a method for preserving a cholesterol oxidase, and a stabilized composition of cholesterol oxidase. A method for stabilizing a cholesterol oxidase and a method for preserving a cholesterol oxidase which comprises allowing the cholesterol oxidase to coexist with an α-keto acid in an aqueous medium, and, a stabilized composition of cholesterol oxidase which comprises the cholesterol oxidase being allowed to coexist with an α-keto acid in an aqueous medium. The method for stabilizing a cholesterol oxidase, the method for preserving a cholesterol oxidase, and a stabilized composition of cholesterol oxidase according to the present invention are useful for clinical diagnosis such as metabolic syndrome.
1. A method for stabilizing a cholesterol oxidase which comprises the steps of:
preparing an aqueous solution of cholesterol oxidase;
adding an α-keto acid or a salt thereof to the aqueous medium to prepare an aqueous solution comprising the α-keto acid or a salt thereof and the cholesterol oxidase; and
preserving the aqueous solution comprising the α-keto acid or a salt thereof and the cholesterol oxidase at −5 to 45° C. for at least two weeks, wherein
a concentration of the α-keto acid or a salt thereof in said aqueous solution is from 0.05 to 40 mmol/L, and
the α-keto acid is selected from the group consisting of pyruvic acid, α-ketoglutaric acid and oxaloacetic acid.
2. The method for stabilizing a cholesterol oxidase according to claim 1 , wherein said cholesterol oxidase is isolated.