Alphavirus compositions and methods of use
Embodiments are directed compositions related to Eilat virus and uses thereof.
1. A recombinant alphavirus expression cassette comprising (i) an alphavirus nucleic acid segment having a nucleic acid sequence that is at least 95% identical to the nucleic acid sequence of at least 100 consecutive nucleotides of SEQ ID NO: 1 and (ii) a heterologous nucleic acid segment.
2. The expression cassette of claim 1 , wherein the alphavirus nucleic acid segment has a nucleic acid sequence that is the nucleic acid sequence of at least 100 consecutive nucleotides of SEQ ID NO: 1.
3. The expression cassette of claim 1 , wherein the expression cassette is comprised in a pRS2 plasmid backbone.
4. A host cell comprising an expression vector of claim 1 .
5. The expression cassette of claim 1 , wherein the heterologous nucleic acid segment encodes heterologous alphavirus structural proteins.
6. An alphavirus produced by the cell of claim 4 .
7. An immunogenic composition comprising the alphavirus of claim 6 .
8. A method of stimulating an immune response in a subject comprising administering an effective amount of an immunogenic composition of claim 7 .