Frictional trans-epithelial tissue disruption and collection apparatus and method of inducing or augmenting an immune response
The invention relates to trans-epithelial frictionally abrasive tissue sampling devices for performing biopsies and methods of inducing an immune response against a pathogen, wherein epithelial cells containing the pathogen are disrupted with the frictionally abrasive tissue sampling device to introduce the pathogen into the bloodstream of a patient.
1. A method of inducing or augmenting an immune response comprising:
frictionally disrupting one or more epithelial cells containing a virus in a region with an apparatus for obtaining a histological sample comprising:
a stiff handle;
a platform at a distal end of the stiff handle; and
a fabric attached to the platform for obtaining a histological sample including:
a backing material securely attached to the platform; and
a plurality of fenestrated loops of a length attached to the backing material, where
the plurality of fenestrated loops have a hook end, where a distance from the top of the plurality of fenestrated loops to the bottom of the hook end is less than approximately 20% of the length of the plurality of fenestrated loops, where the stiff handle does not permit the apparatus to bend or bow when pressure is transmitted from the stiff handle to the platform; and
thereby eliciting a viral immune response in the region.
2. The method of claim 1 , where disruption of the one or more epithelial cells results in one or both the virus and its molecular components being released to surrounding tissue and introduced into the bloodstream of a patient.
3. The method of claim 1 , where the one or more epithelial cells are located at a lesion site.
4. The method of claim 3 , where the lesion site is a mucosal lesion site.
5. The method of claim 1 , where the virus contains a DNA fragment.
6. The method of claim 1 , where the virus contains a virally altered DNA.
7. The method of claim 1 , where the virus contains a protein.
8. The method of claim 7 , where the virus contains an oncogenic protein.
9. The method of claim 8 , where the virus contains a cellular oncogenic protein.
10. The method of claim 1 , where the virus contains an antigenic material.
11. The method of claim 10 , where the antigenic material generates a humoral response.
12. The method of claim 10 , where the antigenic material generates an antibody response.
13. The method of claim 10 , where the antigenic material generates a cell mediated response.
14. The method of claim 10 , where the antigenic material generates one or more of a dendritic cell (DCs), natural killer (NK) cell, macrophage and T lymphocyte response.
15. A method of inducing an immune response against a viral pathogen that normally evades the immune system comprising:
disrupting a cervical epithelial tissue containing the viral pathogen in a region with an apparatus for obtaining a histological sample comprising:
a stiff handle;
a platform at a distal end of the stiff handle; and
a fabric attached to the platform for obtaining a histological sample including:
a backing material securely attached to the platform; and
a plurality of fenestrated loops of a length attached to the backing material, where the plurality of fenestrated loops have a hook end, where a distance from the top of the plurality of fenestrated loops to the bottom of the hook end is less than approximately 20% of the length of the plurality of fenestrated loops, where the stiff handle does not permit the apparatus to bend or bow when pressure is transmitted from the stiff handle to the platform; and
thereby eliciting a viral immune response in the region.
16. The method of claim 15 , where disruption of the cervical epithelial tissue results in the viral pathogen being introduced into the bloodstream of a patient.
17. The method of claim 15 , where the cervical epithelial tissue is located at a lesion site.
18. A method of inducing a viral immune response comprising:
disrupting one or more cervical epithelial tissue cells containing a virus in a region with an apparatus for obtaining a histological sample comprising:
a stiff handle;
a platform at a distal end of the stiff handle; and
a fabric attached to the platform for obtaining a histological sample including:
a backing material securely attached to the platform; and
a plurality of fenestrated loops of a length attached to the platform, where the plurality of fenestrated loops have a hook end, where a distance from the top of the plurality of fenestrated loops to the bottom of the hook end is less than approximately 20% of the length of the plurality of fenestrated loops, where the stiff handle does not permit the apparatus to bend or bow when pressure is transmitted from the stiff handle to the platform; and
thereby eliciting a viral immune response in the region.
19. The method of claim 18 , where disruption of the one or more cervical epithelial tissue cells results in the virus being introduced into the bloodstream of a patient.
20. The method of claim 18 , where the one or more cervical epithelial tissue cells are located at a lesion site.