Methods of introducing nucleic acids into cellular DNA
A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.
1. A method of introducing a nucleic acid sequence into the genome of a cell where the cell has impaired or inhibited or disrupted DnaG primase activity, or impaired or inhibited or disrupted DnaB helicase activity, comprising transforming the cell with a nucleic acid oligomer, wherein the nucleic acid oligomer is introduced into the genome of the cell through recombination, and wherein the nucleic acid oligomer is single-stranded DNA.
2. The method of claim 1 wherein the cell is transformed with multiple nucleic acid oligomers.
3. The method of claim 1 , wherein multiple mutations are generated in a chromosome of the cell through recombination.
4. The method of claim 1 , wherein multiple mutations are generated in the genome of the cell through recombination.
5. The method of claim 1 , wherein the cell is contacted with a pool of nucleic acid oligomers.
6. The method of claim 1 wherein the cell is deficient in at least one nuclease.
7. The method of claim 1 wherein the cell is grown into a population of cells having impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, and the population of cells is transformed with at least one nucleic acid oligomer.
8. The method of claim 1 wherein the cell is grown into a population of cells having impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, and the population of cells is transformed with at least one nucleic acid oligomer and the steps of growing and transforming are repeated until a plurality of nucleic acid sequences have been introduced into the cells.
9. A method of serially introducing a nucleic acid sequence into the genome of a cell where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, comprising transforming the cell with a nucleic acid oligomer two or more times, wherein the nucleic acid oligomer is introduced into the genome of the cell through recombination, and wherein the nucleic acid oligomer is single-stranded DNA.
10. The method of claim 9 wherein the cell is transformed with multiple nucleic acid oligomers.
11. The method of claim 9 , wherein multiple mutations are generated in a chromosome of the cell through recombination.
12. The method of claim 9 , wherein multiple mutations are generated in the genome of the cell through recombination.
13. The method of claim 9 , wherein the cell is contacted with a pool of nucleic acid oligomers.
14. The method of claim 9 wherein the cell is deficient in at least one nuclease.
15. The method of claim 9 wherein the cell is grown into a population of cells having impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, and the population of cells is transformed with at least one nucleic acid oligomer.
16. The method of claim 9 wherein the cell is grown into a population of cells having impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, and the population of cells is transformed with at least one nucleic acid oligomer and the steps of growing and transforming are repeated until a plurality of nucleic acid sequences have been introduced into the cells.
17. A method of introducing a nucleic acid sequence into the genome of a cell where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, and is deficient in at least one nuclease comprising transforming the cell with a nucleic acid oligomer, wherein the nucleic acid oligomer is introduced into the genome of the cell through recombination, and wherein the nucleic acid oligomer is single-stranded DNA.
18. The method of claim 17 wherein a plurality of eXogenous nucleic acid sequences are introduced through recombination into the genome of the cells having impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, and being deficient in at least one nuclease.