Transgenic plant event detection

1. A kit for examining a sample for the presence or absence of material derived from one or more transgenic plant events, the kit comprising primer pairs for amplification of one, more than one or all of nucleic acids chosen from:

a) “Zm”: a nucleic acid derived from and specific for Zea mays taxon,

b) “Bn”: a nucleic acid derived from and specific for Brassica napus taxon,

c) “Gm”: a nucleic acid derived from and specific for Glycine max taxon,

o) “Or”: a nucleic acid derived from and specific for Oryza sativa taxon,

p) “Bv”: a nucleic acid derived from and specific for Beta vulgaris taxon,

q) “Gs”: a nucleic acid derived from and specific for Gossypium taxon, and

t) “St”: a nucleic acid derived from and specific for Solanum tuberosum taxon; and

primer pairs for amplification of all of nucleic acids d)-i):

d) “p35S”: a nucleic acid derived from the 35S promoter of Cauliflower Mosaic Virus,

e) “tNOS”: a nucleic acid derived from the 3′ terminator of the Agrobacterium tumefaciens nopaline synthetase gene,

f) “Cry1Ab”: a nucleic acid derived from the crystal protein gene Cry1Ab of Bacillus thuringiensis,

g) “PAT/bar”: a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene bar of Streptomyces hygroscopicus,

h) “PAT/pat”: a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene pat of Streptomyces viridochromogenes , and

i) “CP4-EPSPS”: a nucleic acid derived from the 5-Enol-pyruvylshikimate-3-phosphate synthase EPSPS gene from Agrobacterium sp. CP4;

wherein the respective primer pairs are from the following table, or variants showing at least 85% sequence identity to said primer pairs:

“Zm”

Fwd: 5′ TCTCTTCCTCCTTTAGAGCTACCACTA 3′

(SEQ ID NO: 56)

Rev: 5′ AATCGATCCAAAGCGAGATGA 3′

(SEQ ID NO: 57)

“Bn”

Fwd: 5′ CAGCTCAACAGTTTCCAAACGA 3′

(SEQ ID NO: 24)

Rev: 5′ CGACCAGCCTCAGCCTTAAG 3′

(SEQ ID NO: 25)

“Gm”

Fwd: 5′ AACCGGTAGCGTTGCCAG 3′

(SEQ ID NO: 58)

Rev′: 5′ AGCCCATCTGCAAGCCTTT 3′

(SEQ ID NO: 59)

“p35S”

Fwd: 5′ AAAGCAAGTGGATTGATGTGATA 3′

(SEQ ID NO: 60)

Rev: 5′ GGGTCTTGCGAAGGATAGTG 3′

(SEQ ID NO: 61)

“tNOS”

Fwd: 5′-GATTAGAGTCCCGCAATTATACATTTAA-3′

(SEQ ID NO: 9)

Rev: 5′-TTATCCTAGKTTGCGCGCTATATTT-3′

(SEQ ID NO: 10)

“Cry1Ab”

Fwd: 5′-ACCGGTTACACTCCCATCGA-3′

(SEQ ID NO: 11)

Rev: 5′-CAGCACCTGGCACGAACTC-3′

(SEQ ID NO: 12)

“PAT/bar”

Fwd: 5′-CGTCAACCACTACATCGAGACAA-3′

(SEQ ID NO: 13)

Rev: 5′-GTCCACTCCTGCGGTTCCT-3′

(SEQ ID NO: 14)

“PAT/pat”

Fwd: 5′-CCGCGGTTTGTGATATCGTT-3′

(SEQ ID NO: 15)

Rev: 5′-TCTTGCAACCTCTCTAGATCATCAA-3′

(SEQ ID NO: 16)

“CP4-EPSPS”

Fwd: 5′ GCATGCTTCACGGTGCAA 3′

(SEQ ID NO: 22)

Rev: 5′ GGACCTGTGGGAGATAGACTTGTC 3′

(SEQ ID NO: 23)

Rev 1: 5′ TGAAGGACCGGTGGGAGAT 3′

(SEQ ID NO: 62)

Rev 2: 5′ TGAAGGACCTGTGGGAGAT 3′

(SEQ ID NO: 63)

“Or”

Fwd′: 5′ GCTTAGGGAACAGGGAAGTAAAGT 3′

(SEQ ID NO: 51)

Rev: 5′ CTTAGCATAGTCTGTGCCATCCA 3′

(SEQ ID NO: 21)

“Bv”

Fwd: 5′ GACCTCCATATTACTGAAAGGAAG 3′

(SEQ ID NO: 64)

Rev: 5′ GAGTAATTGCTCCATCCTGTTCA 3′

(SEQ ID NO: 65)

“Gs”

Fwd: 5′ AGTTTGTAGGTTTTGATGTTACATTGAG 3′

(SEQ ID NO: 66)

Rev: 5′ GCATCTTTGAACCGCCTACTG 3′

(SEQ ID NO: 67)

“St”

Fwd: 5′ GGACATGTGAAGAGACGGAGC 3′

(SEQ ID NO: 68)

Rev: 5′ CCTACCTCTACCCCTCCGC 3′

(SEQ ID NO: 69)

and wherein at least one primer of the primer pairs is labelled.

2. The kit according to claim 1 , further comprising one or more nucleic acids suitable as a positive control for amplification of one, more than one or all nucleic acids chosen from:

a) “Zm”: a nucleic acid derived from and specific for Zea mays taxon,

b) “Bn”: a nucleic acid derived from and specific for Brassica napus taxon,

c) “Gm”: a nucleic acid derived from and specific for Glycine max taxon,

o) “Or”: a nucleic acid derived from and specific for Oryza sativa taxon,

p) “Bv”: a nucleic acid derived from and specific for Beta vulgaris taxon,

q) “Gs”: a nucleic acid derived from and specific for Gossypium taxon, and

t) “St”: a nucleic acid derived from and specific for Solanum tuberosum taxon; and

amplification of all of nucleic acids d)-i):

d) “p35S”: a nucleic acid derived from the 35S promoter of Cauliflower Mosaic Virus,

e) “tNOS”: a nucleic acid derived from the 3′ terminator of the Agrobacterium tumefaciens nopaline synthetase gene,

f) “Cry1Ab”: a nucleic acid derived from the crystal protein gene Cry1Ab of Bacillus thuringiensis,

g) “PAT/bar”: a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene bar of Streptomyces hygroscopicus,

h) “PAT/pat”: a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene pat of Streptomyces viridochromogenes , and

i) “C P4-EPSPS”: a nucleic acid derived from the 5-Enol-pyruvylshikimate-3-phosphate synthase EPSPS gene from Agrobacterium sp. CP4;

wherein the respective primer pairs are from the following table, or variants showing at least 85% sequence identity to said primer pairs:

“Zm”

Fwd: 5′ TCTCTTCCTCCTTTAGAGCTACCACTA 3′ (SEQ ID NO: 56)

Rev: 5′ AATCGATCCAAAGCGAGATGA 3′ (SEQ ID NO: 57)

“Bn”

Fwd: 5′ CAGCTCAACAGTTTCCAAACGA 3′ (SEQ ID NO: 24)

Rev: 5′ CGACCAGCCTCAGCCTTAAG 3′ (SEQ ID NO: 25)

“Gm”

Fwd: 5′ AACCGGTAGCGTTGCCAG 3′ (SEQ ID NO: 58)

Rev′: 5′ AGCCCATCTGCAAGCCTTT 3′ (SEQ ID NO: 59)

“p35S”

Fwd: 5′ AAAGCAAGTGGATTGATGTGATA 3′ (SEQ ID NO: 60)

Rev: 5′ GGGTCTTGCGAAGGATAGTG 3′ (SEQ ID NO: 61)

“tNOS”

Fwd: 5′-GATTAGAGTCCCGCAATTATACATTTAA-3′ (SEQ ID NO: 9)

Rev: 5′-TTATCCTAGKTTGCGCGCTATATTT-3′ (SEQ ID NO: 10)

“Cry1Ab”

Fwd: 5′-ACCGGTTACACTCCCATCGA-3′ (SEQ ID NO: 11)

Rev: 5′-CAGCACCTGGCACGAACTC-3′ (SEQ ID NO: 12)

“PAT/bar”

Fwd: 5′-CGTCAACCACTACATCGAGACAA-3′ (SEQ ID NO: 13)

Rev: 5′-GTCCACTCCTGCGGTTCCT-3′ (SEQ ID NO: 14)

“PAT/pat”

Fwd: 5′-CCGCGGTTTGTGATATCGTT-3′ (SEQ ID NO: 15)

Rev: 5′-TCTTGCAACCTCTCTAGATCATCAA-3′ (SEQ ID NO: 16)

“CP4-EPSPS”

Fwd: 5′ GCATGCTTCACGGTGCAA 3′ (SEQ ID NO: 22)

Rev: 5′ GGACCTGTGGGAGATAGACTTGTC 3′ (SEQ ID NO: 23)

Rev 1: 5′ TGAAGGACCGGTGGGAGAT 3′ (SEQ ID NO: 62)

Rev 2: 5′ TGAAGGACCTGTGGGAGAT 3′ (SEQ ID NO: 63)

“Or”

Fwd′: 5′ GCTTAGGGAACAGGGAAGTAAAGT 3′ (SEQ ID NO: 51)

Rev: 5′ CTTAGCATAGTCTGTGCCATCCA 3′ (SEQ ID NO: 21)

“Bv”

Fwd: 5′ GACCTCCATATTACTGAAAGGAAG 3′ (SEQ ID NO: 64)

Rev: 5′ GAGTAATTGCTCCATCCTGTTCA 3′ (SEQ ID NO: 65)

“Gs”

Fwd: 5′ AGTTTGTAGGTTTTGATGTTACATTGAG 3′ (SEQ ID NO: 66)

Rev: 5′ GCATCTTTGAACCGCCTACTG 3′ (SEQ ID NO: 67)

“St”

Fwd: 5′ GGACATGTGAAGAGACGGAGC 3′ (SEQ ID NO: 68)

Rev: 5′ CCTACCTCTACCCCTCCGC 3′ (SEQ ID NO: 69)

and wherein at least one primer of the primer pairs is labelled.

3. The kit according to claim 1 , further comprising a primer pair for amplification of a generic plant-derived nucleic acid.

4. The kit according to claim 3 , wherein the generic plant-derived nucleic acid is a nucleic acid derived from the chloroplastic small subunit of Rubisco gene or from the CHL-tRNA synthetase gene.

5. The kit according to claim 3 , wherein the primer pair for amplification of the generic plant-derived nucleic acid is the primer pair 5′ AGGTCTAADGGRTAAGCTAC 3′ (SEQ ID NO: 26) and 5′ AGYCTTGATCGTTACAAAGG 3′ (SEQ ID NO: 27), or a variant showing at least 85% sequence identity to said primer pair.

6. The kit according to claim 1 , wherein at least one primer of a primer pair comprises a fluorophore label.

7. The kit according to claim 1 , wherein the Zea mays taxon is Zea mays ssp. Mays.

8. The kit according to claim 5 , wherein at least one primer of a primer pair comprises a fluorophore label.

9. The kit according to claim 2 , wherein the Zea mays taxon is Zea mays ssp. Mays.