Multiplex slide plate
The present invention relates to a multiplex slide plate for various types of assays. The slide plate may be pre-filled with special-formulated reagents in different reaction zones, and the reactions carry out independently in the reaction zones filled with special-formulated reagent.
1. A multiplex slide plate, comprising:
a slide plate having a plurality of reaction vessels, wherein the plurality of reaction vessels is arranged in arrays and each of the plurality of reaction vessels has an opening portion and a bottom portion, and
wherein each of the plurality of reaction vessels includes a formulation of a mixture of a prefilled reagent and a controlled-release substance, and the formulation of the mixture of the prefilled reagent and the controlled-release substance allows a delayed release of the prefilled reagent during a period of filling a sample solution to the reaction vessel, and the controlled-release substance includes glycerol, poly(vinyl alcohol) (PVA), poly(vinyl pyrrolidone) (PVP), polyethylene glycol/polyethylene oxide (PEG/PEO), alginic acid, natural starch, artificial starch, polyurethane, micron particles of activated carbon or nano-particles of activated carbon.
2. The multiplex slide plate as claimed in claim 1 , wherein a formulation of the prefilled reagent includes a pair of primers of a polymerase chain reaction (PCR).
3. The multiplex slide plate as claimed in claim 2 , wherein the formulation of the prefilled reagent further includes a reporter.
4. The multiplex slide plate as claimed in claim 1 , wherein a content of glycerol is 30 wt % or more and a formulation of the prefilled reagent becomes viscous with a gravity sliding rate less than 5 μm/day.
5. The multiplex slide plate as claimed in claim 1 , wherein the slide plate is made of polycarbonate (PC) or poly(methyl methacrylate) (PMMA).
6. The multiplex slide plate as claimed in claim 1 , wherein each of the plurality of reaction vessels has a slanted sidewall connecting the opening portion and the bottom portion and has an angle θ of greater than 90 degrees, more preferred between 100 to 135 degrees, more preferred of 110 to 120 degrees, relative to the bottom portion.
7. The multiplex slide plate as claimed in claim 1 , wherein the slide plate includes a sample loading region.
8. The multiplex slide plate as claimed in claim 1 , further comprising a cover over the slide plate to form a close space between the slide plate and the cover.
9. The multiplex slide plate as claimed in claim 1 , wherein each of the plurality of reaction vessels has two open ends.
10. The multiplex slide plate as claimed in claim 1 , wherein each of the plurality of reaction vessels has two open ends, and a slanted sidewall connecting the two open ends.