Single particle analyzer and single particle analysis method
According to one embodiment, provided is a single particle analyzing device including a measuring vessel, first and second chambers in the vessel defined by an insulating membrane, a pore opening in the membrane to connect the chambers, and first and second electrodes in the chambers. Electric current flows between the electrodes through the pore. Electrical characteristics are measured during migration of the target from the first chamber to the second chamber to measure the size and shape of the target. (a) t<a <d≦100 a or (b) s<L, s<d≦100 s , t<L and t<d, wherein a, L and s are the diameter, length and width of the target, d is the diameter of the pore, and t is the thickness of the membrane in the proximity to the pore.
1. A single particle analyzing device comprising:
a measuring vessel;
a first chamber and a second chamber in the measuring vessel defined by an insulating membrane;
a pore opening in the membrane to connect the first chamber to the second chamber; and
a first electrode in the first chamber and a second electrode in the second chamber,
wherein the analyzing device is configured to measure a shape of a target by applying electric current between the first and second electrodes through the pore in the membrane and measuring a signal detected during translocation of the target from the first chamber to the second chamber through the pore, and
wherein the analyzing device satisfies the following dimensional relationships:
t<d
where d is a diameter of the pore, which is in a range of greater than or equal to 50 nm and less than or equal to 1 mm, the diameter d is greater than a diameter of the target, and t is a thickness of the membrane in the proximity to the pore, which is in a range of greater than or equal to 10 nm and less than or equal to 500 nm, the thickness t being less than the diameter of the target.
2. The single particle analyzing device according to claim 1 , wherein the target is a particle having diameter which is in a range of greater than 10 nm and less than 1 mm.
3. The single particle analyzing device according to claim 1 , wherein the target is selected from the group consisting of pollen, bacterium, and virus.
4. The single particle analyzing device according to claim 1 , wherein the first and second electrodes are not in contact with the membrane.
5. The single particle analyzing device according to claim 1 , wherein the membrane comprises a first surface and a second surface, the first surface being contacted with the first electrode in the first chamber, and the second surface being contacted with the second electrode in the second chamber.
6. A single particle analysis method, comprising:
(1) preparing a single particle analyzing device comprising a measuring vessel, a first chamber and a second chamber in the measuring vessel defined by an insulating membrane, a pore opening in the membrane to connect the first chamber to the second chamber, and a first electrode in the first chamber and a second electrode in the second chamber, and
wherein the analyzing device satisfies the following dimensional relationships:
t<d
where d is a diameter of the pore, which is in a range of greater than or equal to 50 nm and less than or equal to 1 mm, the diameter d is greater than a diameter of a target, and t is a thickness of the membrane in the proximity to the pore, which is in a range of greater than or equal to 10 nm and less than or equal to 500 nm, the thickness t being less than the diameter of the target,
(2) charging a conductive liquid into the first and second chambers;
(3) introducing targets into the first chamber; and
(4) measuring a shape of the target by applying electric current between the first and second electrodes through the pore in the membrane and measuring a signal detected during translocation of the target from the first chamber to the second chamber through the pore.
7. The single particle analysis method according to claim 6 , wherein electricity is supplied between the first and second electrodes by applying a constant voltage, and the signal detected is an electric current.
8. The single particle analysis method according to claim 6 , wherein the target is a particle having diameter which is in a range of greater than 10 nm and less than 1 mm.
9. The single particle analysis method according to claim 6 , wherein the target is selected from the group consisting of pollen, bacterium, and virus.
10. The single particle analysis method according to claim 6 , wherein the translocation of the target from the first chamber to the second chamber through the pore is promoted by electrophoresis of the target from the first chamber to the second chamber.
11. The single particle analysis method according to claim 6 , wherein the translocation of the target from the first chamber to the second chamber through the pore is promoted by liquid flow from the first chamber to the second chamber.
12. The single particle analysis method according to claim 11 , wherein the liquid flow is an electroosmotic flow.
13. The single particle analysis method according to claim 11 , wherein the liquid flow is generated by a pressure difference between the first chamber and the second chamber.
14. The single particle analysis method according to claim 6 , wherein the first and second electrodes are not in contact with the membrane.
15. The single particle analysis method according to claim 6 , wherein the membrane has a first surface and a second surface, the first surface being contacted with the first electrode in the first chamber, and the second surface being contacted with the second electrode in the second chamber.