Plants having altered expression and activity of yield-related proteins
Transgenic plants that have enhanced yield-related traits, such as increased seed oil production, are produced by genetically engineering the plants to down-regulate the expression of at least one BPM protein. Such transgenic plants can, for example, be cultivated and yield higher seed oil production than control plants which have not been genetically engineered for down regulation of a BPM protein.
1. A transgenic plant, wherein said plant is genetically engineered to express a nucleic acid so as to down-regulate expression or reduce the activity of all of the members of the BPM (BTB/POZ-MATH) protein family as compared to a control plant, wherein said transgenic plant exhibits enhanced yield-related traits as compared to said control plant, and wherein said enhanced yield-related traits are selected from the group consisting of increased seed oil production, increased salt stress-tolerance, and increased drought stress-tolerance as compared to said control plant.
2. The transgenic plant of claim 1 , wherein said plant is of the Brassicaceae family.
3. The transgenic plant of claim 2 , wherein said plant is Arabidopsis Thaliana.
4. The transgenic plant of claim 1 , wherein said transgenic plant exhibits increased seed oil production as compared to said control plant.
5. A method for increasing seed oil production in a transgenic plant as compared to a control plant and recovering said seed oil, said method comprising: cultivating a transgenic plant under conditions promoting plant growth and development, wherein said transgenic plant is genetically engineered to express a nucleic acid so as to exhibit increased seed oil production as compared to a control plant by down-regulating expression or reducing the activity of all of the members of the BPM protein family as compared to a control plant; and recovering said seed oil from said transgenic plant.
6. The method of claim 5 , wherein said plant is of the Brassicaceae family.
7. The method of claim 6 , wherein said plant is Arabidopsis Thaliana.
8. A method for enhancing yield-related traits in a plant as compared to a control plant, said method comprising: genetically engineering said plant to express a nucleic acid so as to down-regulate expression or reduce the activity of all of the members of the BPM protein family as compared to a control plant, wherein said yield-related traits are selected from the group consisting of increased seed oil production, increased salt stress-tolerance, and increased drought stress-tolerance as compared to said control plant, and testing the genetically engineered plant to assess a yield-related trait selected from the group consisting of seed oil production, salt stress-tolerance, and drought stress-tolerance.
9. The method of claim 8 , wherein said plant exhibits increased seed oil production as compared to a control plant.
10. The method of claim 8 , wherein said plant is of the Brassicaceae family.
11. The method of claim 10 , wherein said plant is Arabidopsis Thaliana.
12. The method of claim 8 , wherein said step of genetically engineering comprises introducing artificial microRNA (amiRNA) to down-regulate all of the members of the BPM protein family.
13. A method for enhancing yield-related traits in a plant as compared to a control plant, said method comprising: genetically engineering said plant to express a nucleic acid so as to reduce the activity of at least one BPM protein as compared to a control plant, wherein said yield-related traits are selected from the group consisting of increased seed oil production, increased salt stress-tolerance, and increased drought stress-tolerance as compared to said control plant, wherein said step of genetically engineering comprises the expression of at least one exogenous MATH domain to compete with said BPM protein, and testing the genetically engineered plant to assess a yield-related trait selected from the group consisting of seed oil production, salt stress-tolerance, and drought stress-tolerance.
14. A product produced by or from a transgenic plant which is genetically engineered to express a nucleic acid so as to enhance yield-related traits by down-regulating expression or reducing the activity of all of the members of the BPM protein family as compared to a control plant, wherein said enhanced yield-related traits are selected from the group consisting of increased seed oil production, increased salt stress-tolerance, and increased drought stress-tolerance as compared to said control plant, and wherein the product comprises the expressed nucleic acid.