Compositions and methods for detecting human Pegivirus 2 (HPgV-2)
Provided herein are compositions, methods, and kits for detecting human Pegivirus 2 (HPgV-2). In certain embodiments, provided herein are HPgV-2 specific nucleic acid probes and primers, and methods for detecting HPgV-2 nucleic acid. In other embodiments, provided herein are HPgV-2 immunogenic composition compositions, methods of treating a subject with immunogenic HPgV-2 peptides, and methods of detecting HPgV-2 subject antibodies in a sample.
1. A method of detecting human Pegivirus 2 (HPgV-2) specific antibodies in a subject comprising:
a) contacting a sample from a subject suspected of containing an antibody to HPgV-2 with a HPgV-2 peptide, wherein said peptide specifically binds said antibody to form a complex, and
wherein said HPgV-2 peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:86-99 and 100-218; and
b) detecting whether said antibody is present in said sample by detecting binding between said antibody and said peptide.
2. The method of claim 1 , wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs: 129-132.
3. The method of claim 1 , wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs: 163-166.
4. The method of claim 1 , wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs: 209-212.
5. The method of claim 1 , wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs: 213-218.
6. The method of claim 1 , where said amino acid sequence is selected from the group consisting of SEQ ID NOs: 209-218.
7. The method of claim 1 , wherein said HPgV-2 peptide comprises a label.
8. The method of claim 7 , further comprising contacting said sample with a solid support comprising moieties that bind said label.
9. The method of claim 8 , further comprising incubating said sample under conditions such that: i) said HPgV-2 peptide specifically binds said antibody to form a complex, and ii) said complex binds to said solid support via said label binding at least one of said moieties.
10. The method of claim 9 , further comprising washing said solid support.
11. The method of claim 10 , further comprising adding to said sample a detectably labeled secondary antibody capable of binding the antibody in said complex.