Dual path immunoassay device
Systems include test cells with sorbent material in a T-shape and defining a first flow path for a solution and a second flow path for a sample, and a test line or test site with immobilized antigens or antibodies or other ligand binding molecules located at the junction of the T. A housing houses the sorbent material and defines a first hole adjacent an end of the first flow path for receiving the solution and a second hole adjacent an end of the second flow path for receiving the sample.
1. A test device for use with a solution and for determining the presence of a ligand in a liquid sample, the test device comprising:
a first sorbent strip having a first end defining a solution receiving portion that receives the solution, and having a test site displaced from said first end, said test site having an immobilized ligand-binding mechanism thereon; and
a second sorbent strip perpendicular to said first sorbent strip, said second sorbent strip having a first end defining a sample receiving portion that receives the liquid sample, and a portion displaced from said first end of said second sorbent strip and touching said first sorbent strip at or adjacent said test site such that the liquid sample applied to said first end of said second sorbent strip laterally migrates over time to said first sorbent strip and does not immediately wet said first sorbent strip.
2. A test device according to claim 1 , wherein:
said first sorbent strip supports a conjugate having a marker between said first end and said test site.