Anti-fugetactic agents for the treatment of ovarian cancer
This invention provides methods and compositions for modulating movement of eukaryotic cells with migratory capacity. More specifically, the invention provides anti-fugetactic agents and methods for the use thereof in enhancing an immune response.
1. A method for increasing the migratory capacity of immune cells into a mammalian ovarian tumor exhibiting fugetactic activity on said immune cells due to CXCL12 secretion, wherein said immune cells comprise tumor antigen-specific T-cells, which method comprises:
a) selecting a mammal having said tumor which secretes a fugetactic effective amount of CXCL12 around said tumor wherein said concentration of CXCL12 is greater than 100 nM;
b) contacting said T-cells with an anti-fugetactic concentration of 1,1′-[1,4-phenylenebis-(methylene)]bis[1,4,8,11-tetraazacyclotetradecane] of from 0.08 μg/mL up to 5 μg/mL wherein said T-cells are now capable of increasing their migration into said tumor through the fugetactic activity of said tumor.
2. The method of claim 1 , wherein said method further comprises administering anti-cancer therapy to the said patient.
3. The method of claim 2 , wherein said anti-cancer therapy is a chemotherapeutic agent selected from the group consisting of paclitaxel, cisplatin, and doxorubicin.
4. The method of claim 3 , wherein the chemotherapeutic agent is paclitaxel.
5. The method of claim 1 , wherein the anti-fugetactic agent is in a sustained release formulation.
6. The method of claim 1 , wherein the mammal is a human.