Compositions and methods relating to nucleic acid nano- and micro-technology
The invention provides nucleic acid structures of controlled size and shape, comprised of a plurality of oligonucleotides, and methods for their synthesis. The structures are formed, at least in part, by the self-assembly of single stranded oligonucleotides. The location of each oligonucleotide in the resultant structure is known. Accordingly, the structures may be modified with specificity.
1. A nucleic acid structure comprising
a plurality of oligonucleotides, wherein at least one oligonucleotide of the plurality comprises at least four domains, wherein each of the four domains is annealed to a complementary domain of another oligonucleotide of the plurality to form at least two parallel double helices within the nucleic acid structure, wherein oligonucleotides having single-stranded poly(T), poly(A) or poly(C) domains that each have a length of 4-22 nucleotides are present at borders around the nucleic acid structure.
2. The nucleic acid structure of claim 1 , wherein at least one double helix within the nucleic acid structure comprises 2 or more unique domains.
3. The nucleic acid structure of claim 1 , wherein at least 50% of the double helices within the nucleic acid structure comprise one or more unique domains.
4. The nucleic acid structure of claim 1 , wherein the nucleic acid structure comprises at least 5, at least 10, or at least 20 parallel double helices.
5. The nucleic acid structure of claim 1 , wherein a first subset of oligonucleotides of the plurality comprises 2 domains and a second subset of oligonucleotides of the plurality comprises 4 domains.
6. The nucleic acid structure of claim 1 , wherein the oligonucleotides are 21-104 nucleotides in length.
7. The nucleic acid structure of claim 1 , wherein the oligonucleotides are DNA oligonucleotides.
8. The nucleic acid structure of claim 1 , wherein the oligonucleotides are L-DNA oligonucleotides.
9. The nucleic acid structure of claim 1 , wherein at least one of the double helices within the nucleic acid structure is unique.
10. The nucleic acid structure of claim 9 , wherein the nucleic acid structure comprises 2 or more unique double helices.
11. The nucleic acid structure of claim 9 , wherein at least 50% of the double helices are unique.
12. The nucleic acid structure of claim 9 , wherein at least 50% of the double helices comprise one or more unique domains.
13. The nucleic acid structure of claim 9 , wherein the nucleic acid structure comprises at least 5, at least 10, or at least 20 parallel double helices.
14. The nucleic acid structure of claim 9 , wherein a first subset of oligonucleotides of the plurality comprises 2 domains and a second subset of oligonucleotides of the plurality comprises 4 domains.
15. The nucleic acid structure of claim 1 , wherein at least one oligonucleotide in the nucleic acid structure is unique.
16. A composition comprising a plurality of nucleic acid structures of claim 1 , wherein at least 50% of the nucleic acid structures of the plurality are identical.
17. A composite nucleic acid structure comprising at least two nucleic acid structures of claim 1 , conjugated to each other through a spacer-linker.
18. The nucleic acid structure of claim 1 , wherein the nucleic acid structure has an internal void pattern.
19. The nucleic acid structure of claim 1 , wherein the nucleic acid structure further comprises oligonucleotides having a handle domain.
20. The nucleic acid structure of claim 19 , wherein the oligonucleotides having a handle domain are present at borders of the nucleic acid structure.
21. The nucleic acid structure of claim 19 , wherein the handle domain is bound to an oligonucleotide conjugated to a moiety of interest.
22. The nucleic acid structure of claim 1 , wherein the single-stranded poly(T), poly(A) or poly(C) domains have a length of 10 nucleotides.
23. The nucleic acid structure of claim 1 , wherein the single-stranded poly(T), poly(A) or poly(C) domains have a length of 11 nucleotides.