Polymers and DNA copolymer coatings
Some embodiments described herein relate to new polymer coatings for surface functionalization and new processes for grafting pre-grafted DNA-copolymers to surface(s) of substrates for use in DNA sequencing and other diagnostic applications.
1. A polymer for surface functionalization, comprising a recurring unit of Formula (I) and a recurring unit of Formula (II):
wherein:
each R 1a , R 2a , R 1b and R 2b is independently selected from the group consisting of hydrogen, optionally substituted alkyl, and optionally substituted phenyl;
each R 3a and R 3b is independently selected from the group consisting of hydrogen, optionally substituted alkyl, optionally substituted phenyl, and optionally substituted C 7-14 aralkyl; and
each L 1 and L 2 is independently selected from the group consisting of an optionally substituted alkylene linker and an optionally substituted heteroalkylene linker.
2. The polymer of claim 1 , wherein R 1a is hydrogen or optionally substituted alkyl and each of R 2a and R 3a is hydrogen.
3. The polymer of claim 1 , wherein R 1b is hydrogen or optionally substituted alkyl and each of R 2b and R 3b is hydrogen.
4. The polymer of claim 1 , wherein L 2 is an optionally substituted alkylene.
5. The polymer of claim 1 , wherein L 1 is an optionally substituted alkylene.
6. The polymer of claim 1 , wherein the recurring unit of Formula (I) is represented by Formula (Ia) and Formula (II) is represented by Formula (IIa):
wherein R 1a is hydrogen or methyl and R 1b is hydrogen or methyl.
7. The polymer of claim 1 , wherein L 1 is an optionally substituted heteroalkylene.
8. The polymer of claim 7 , wherein L 1 is —(CH 2 ) m —NH—(CH 2 ) n — optionally substituted with one or more oxo, and wherein each m and n is independently an integer from 1 to 10.
9. The polymer of claim 1 , wherein the recurring unit of Formula (I) is represented by Formula (Ib):
wherein R 1a is hydrogen or methyl.
10. The polymer of claim 1 , further comprising one or more recurring units selected from the group consisting of polyacrylamides, polyacrylates, polyurethanes, polysiloxanes, silicones, polyacroleins, polyphosphazenes, polyisocyanates, poly-ols, and polysaccharides, and combinations thereof.
11. The polymer of claim 10 , further comprising one or more recurring units of Formula (IIIa) or (IIIb) or both:
wherein each R 4a , R 4b , and R 5b is independently hydrogen or C 1-3 alkyl; and
each R 5a , R 6a , R 6b , and R 7b is independently hydrogen, optionally substituted C 1-6 alkyl, or optionally substituted phenyl.
12. The polymer of claim 11 , wherein each R 4a , R 4b , and R 5b is independently hydrogen or methyl.
13. The polymer of claim 11 , wherein R 6b and R 7b are both hydrogen.
14. The polymer of claim 11 , wherein at least one of R 5a or R 6a is hydrogen.
15. The polymer of claim 11 , wherein at least one of R 5a or R 6a is methyl.
16. The polymer of claim 11 , wherein the one or more recurring units of Formula (IIIa) are represented by (IIIa1), (IIIa2), or (IIIa3):
17. The polymer of claim 11 , wherein the one or more recurring units of Formula (IIIb) are represented by (IIIb1):
18. A substrate having a first surface comprising a polymer of claim 1 covalently bonded thereto.