Isolated human lung progenitor cells and uses thereof
Provided herein are methods and compositions relating, in part, to the generation of human progenitor cells committed to the lung lineage and uses of such cells for treatment of lung diseases/disorders or injury to the lung. Whether an adult stem cell can be isolated from human adult lung remains controversial in the art and at present, methods for isolating and using adult lung stem cells from humans lack reproducibility. Thus, the methods and compositions described herein are advantageous over the present state of knowledge in the art and permit the generation of human lung progenitor cells for treatment, tissue engineering, and screening assays.
1. A composition comprising an isolated and viable human Nkx2.1 positive, Sox2 positive proximal airway multipotent progenitor cell and a decellularized adult human lung scaffold, wherein the proximal airway multipotent progenitor cell is committed to the lung lineage.
2. The composition of claim 1 , wherein the cell is Tuj1 negative and Pax8 negative.
3. The composition of claim 1 , wherein the cell is proliferative and differentiates into an airway basal stem cell, a ciliated cell, a Clara cell, a neuroendocrine cell, or a squamous epithelial cell under chosen differentiation conditions.
4. The composition of claim 1 , wherein the scaffold is implantable in a subject.
5. The composition of claim 1 , wherein the scaffold is biodegradable.
6. The composition of claim 1 , wherein the progenitor cell is not isolated from an embryo, an embryonic stem cell or other cell derived in culture from an embryo.