Flow control in microfluidic systems
Microfluidic systems and methods including those that provide control of fluid flow are provided. Such systems and methods can be used, for example, to control pressure-driven flow based on the influence of channel geometry and the viscosity of one or more fluids inside the system.
1. A method of operating a microfluidic system, comprising:
applying a pressure drop across an inlet and an outlet of a microfluidic system, while carrying out the following steps:
flowing a first fluid at a first flow rate;
flowing a second fluid in the microfluidic system, wherein the first and second fluids have different viscosities, and wherein the first fluid and the second fluid are separated by a separation fluid that is substantially immiscible with the first and second fluids;
flowing the first fluid from a first channel portion to a second channel portion positioned between the inlet and the outlet of the microfluidic system, wherein a fluid path defined by the first channel portion has a larger cross-sectional area than a cross-sectional area of a fluid path defined by the second channel portion;
causing a volumetric flow rate of the first fluid to decrease by a factor of at least 50 in the microfluidic system; and
preventing any of the first fluid from exiting the microfluidic system via the outlet during operation of the microfluidic system.
2. A device comprising:
a microfluidic system comprising:
a first plug comprising a first fluid and a second plug comprising a second fluid contained in the microfluidic system, wherein the first and second plugs are separated by a separation fluid comprising a gas, and wherein the first plug, the second plug, and the separation fluid are present in the microfluidic system prior to first use of the device;
an inlet and an outlet;
an analysis region positioned between the inlet and the outlet;
a microfluidic channel in fluid communication with the analysis region, the microfluidic channel comprising a first channel portion having a first cross-sectional area; and
a flow constriction region positioned downstream of the first channel portion and upstream of the outlet, wherein the flow constriction region has a second cross-sectional area, the second cross-sectional area being at least 10 times smaller than the first cross-sectional area, wherein the flow constriction region has a height and/or a width of less than 75 microns, and wherein the flow constriction region has a length of at least 1 cm.
3. A device as in claim 2 , wherein the second cross-sectional area is less than 50 μm 2 .
4. A device as in claim 2 , wherein the second cross-sectional area is less than 100 μm 2 .
5. A device as in claim 2 , wherein the second cross-sectional area is at least 25 times smaller than the first cross-sectional area.
6. A device as in claim 2 , wherein the flow constriction region has a length of at least 2 CM.
7. A device as in claim 2 , wherein the flow constriction region comprises a serpentine configuration.
8. A device as in claim 2 , comprising a liquid containment region positioned downstream of the flow constriction region, wherein the liquid containment region comprises an absorbent material.
9. A device as in claim 2 , wherein the first channel portion having the first cross-sectional area is upstream of the analysis region.
10. A device as in claim 2 , wherein the first and second plugs of fluid are stored and sealed in a reagent storage area of the device.
11. A device as in claim 10 , wherein the first and second plugs of fluid are liquids.
12. A device as in claim 2 , wherein the first and second plugs are stored and sealed in a microfluidic channel of the device.
13. A device as in claim 12 , wherein the first and second plugs of fluid are liquids.
14. A device as in claim 2 , wherein the first and/or second fluid is a buffer solution.
15. A device as in claim 2 , wherein the first and/or second fluid comprises a reagent for a chemical and/or biological reagent.
16. A device as in claim 9 , wherein the analysis region is a reaction area comprising physisorbed molecules.
17. A device as in claim 2 , comprising an optical detection region associated with the flow constriction region.
18. A device as in claim 17 , comprising a detector associated with the optical detection region, wherein the detector is adapted and arranged to detect a fluid flowing in the flow constriction region.
19. A device as in claim 2 , wherein the second cross-sectional area is at least 25 times smaller than the first cross-sectional area, wherein the flow constriction region has a length of at least 2 cm, and wherein the flow constriction region comprises a serpentine configuration.
20. A device as in claim 2 , wherein the first and second plugs of fluid are stored and sealed in a microfluidic channel of the device, and wherein the first and second plugs of fluid are liquids.
21. A device as in claim 2 , wherein the analysis region is a part of the microfluidic channel comprising the first channel portion.
22. A device as in claim 2 , wherein the analysis region is a part of the constriction region.