Methods for high-throughput in-situ manufacture of user desired 3D polymeric scaffolds
Biomedical devices and methods are disclosed for the development of 3D polymeric scaffolds for cell culture, high throughput screens for biomolecule purification, and generation of bone mimetic materials. The devices may feature multiple geometries, and scaffold generation capabilities include multiple gel types utilizing organic and aqueous phase pregel. Additionally, macroporous and non-macroporous morphologies are possible.
1. A method for generating a three-dimensional polymeric scaffold in high-throughput in-situ utilizing a substrate with a cavity, comprising:
adding monomers and a porogen in said cavity; and
initiating polymerization in-situ to thereby form said scaffold in said substrate cavity.
2. The method of claim 1 , wherein said porogen comprises a salt fusion column and said monomers are added to said column.
3. The method of claim 1 , wherein said porogen is selected from the group consisting of one or more of a salt and a sugar.
4. The method of claim 1 , wherein said cavity is formed to produce said three-dimensional polymeric scaffold in a cuboid or spherical shape.
5. The method of claim 1 , wherein said substrate comprises acrylic.
6. The method of claim 1 , wherein said substrate is selected from the group consisting of one or more of wood, steel, glass, or polypropylene.
7. The method of claim 1 , further including providing a bottom layer of a waterproof film to said substrate.
8. The method of claim 1 , wherein said monomers are selected from the group consisting of one or more of a collagen, a fibronectin, a PLGA, and a peptide gel.
9. The method of claim 1 , further including a step of adding a pseudoaffinity ligand to a surface of a polymerized scaffold.
10. The method of claim 9 , wherein said pseudoaffinity ligand comprises Doxorubicin.
11. A method for generating a polymeric scaffold in high-throughput utilizing at least two substrates that are joined to form one or more cavities and then disjoined to enable the removal of said scaffold, comprising:
adding monomers in said one or more cavities; and
initiating polymerization to thereby form said scaffold, whereby said at least two substrates are disjoined after polymerization to enable removal of said polymeric scaffold.
12. The method of claim 11 , further including with said monomers a porogen selected from the group consisting of one or more of a salt and a sugar.
13. The method of claim 12 , wherein said porogen comprises a salt fusion column.
14. The method of claim 11 , wherein said one or more cavities are formed to produce said three-dimensional polymeric scaffold in a cuboid or spherical shape.
15. The method of claim 11 , wherein said substrates comprises acrylic.
16. The method of claim 1 , wherein said substrates are selected from the group consisting of one or more of wood, steel, glass, or polypropylene.
17. The method of claim 11 , further including providing a bottom layer of a waterproof film to said substrates.
18. The method of claim 11 , wherein said monomers are selected from the group consisting of one or more of a collagen, a fibronectin, a PLGA, and a peptide gel.
19. The method of claim 11 , further including a step of adding a pseudoaffinity ligand to a surface of a polymerized scaffold.
20. The method of claim 19 , wherein said pseudoaffinity ligand comprises Doxorubicin.