Method for measuring coagulation of blood samples using viscoelastic tests (VET)
This disclosure provides a method for measuring coagulation of blood or plasma samples using viscoelastic tests (VET) wherein the measuring is performed in the presence of immobilized endothelial cells.
1. A method for measuring coagulation of a blood or plasma sample comprising performing a viscoelastic test to measure coagulation of said sample, wherein the measuring is performed in the presence of endothelial cells immobilized on a microcarrier.
2. The method of claim 1 , wherein the viscoelastic test is rotation thromboelastometry or thromboelastography.
3. The method of claim 2 , wherein the endothelial cells are immobilized on microbeads.
4. The method of claim 2 , wherein the endothelial cells are immobilized on microbeads comprising dextran.
5. The method of claim 4 , wherein the endothelial cells are outgrowth endothelial cells, human umbilical vein endothelial cells, human dermal microvascular endothelial cells, or human saphenous vein endothelial cells.
6. The method of claim 2 , wherein the endothelial cells are outgrowth endothelial cells, human umbilical vein endothelial cells, human dermal microvascular endothelial cells or human saphenous vein endothelial cells.
7. The method of claim 1 , wherein the endothelial cells are immobilized on microbeads.
8. The method of claim 7 , wherein the endothelial cells are outgrowth endothelial cells, human umbilical vein endothelial cells, human dermal microvascular endothelial cells, or human saphenous vein endothelial cells.
9. The method of claim 7 , wherein the microbeads comprise a collagen matrix to which the endothelial cells are adhered.
10. The method of claim 1 , wherein the endothelial cells are outgrowth endothelial cells, human umbilical vein endothelial cells, human dermal microvascular endothelial cells, or human saphenous vein endothelial cells.
11. The method of claim 1 , wherein the endothelial cells are immobilized on microbeads comprising dextran.
12. The method of claim 1 , wherein the measuring comprises rotating a pin in a cup containing the blood or plasma and the immobilized endothelial cells.
13. The method of claim 1 , wherein the immobilized endothelial cells are suspended in the blood or plasma during the measuring.
14. The method of claim 1 , wherein the measuring is performed in the presence of CaCl 2 .
15. The method of claim 1 , comprising, during the measuring, identifying a point in time of clotting initiation of the blood or plasma sample.
16. The method of claim 1 , further comprising determining a speed of clot formation of the blood or plasma sample.
17. The method of claim 1 , wherein the endothelial cells comprise human umbilical vein endothelial cells.
18. The method of claim 1 , wherein the endothelial cells comprise outgrowth endothelial cells.
19. The method of claim 1 , wherein the endothelial cells are activated.
20. The method of claim 19 , wherein the endothelial cells are activated by incubation with TNFa.
21. The method of claim 1 , wherein said microcarrier comprises glass, polystyrene, polypropylene, acrylamide, porcine gelatin or dextran.