Collagen-based matrices with stem cells
Collagen based-matrices and methods of their use are described. More particularly, collagen-based matrices for differentiating stem cells and progenitor cells, and for producing and isolating blood vessels and vascularized graft constructs are described.
1. A tissue graft composition, said composition comprising:
an engineered, purified collagen-based matrix comprising collagen fibrils, wherein the matrix has a collagen fibril microstructure including fibril-fibril branching; and
a network of lumenized, endothelial cell-lined vessels formed in vitro, said vessels capable of persisting for more than 7 days in vitro;
wherein said collagen fibrils are polymerized from a pig skin collagen source comprising a mixture of type I collagen monomers and oligomers, wherein the concentration of collagen in the polymerization reaction is 0.3 mg/ml to 1 mg/ml, wherein the vessels are formed from endothelial progenitor cells, and wherein the matrix has a shear storage modulus of 40 Pa to 50 Pa and a fibril volume fraction of 5% to 10%; and
wherein the tissue graft composition is produced by engineering the purified collagen-based matrix, and seeding the matrix at a density of 1×10 5 to 5×10 5 endothelial progenitor cells per milliliter to form one or more vessels within the matrix.
2. The tissue graft composition of claim 1 , wherein said matrix has a loss modulus of from about 1 Pa to about 75 Pa.
3. The tissue graft composition of claim 1 , wherein said matrix has a compression modulus of 2,500 Pa to about 18,000 Pa.