Collagen compositions and methods of use
The present disclosure provides an engineered collagen composition comprising collagen, wherein the collagen composition is compressed to form a gradient of at least one physical property. Methods of using and of manufacturing the engineered collagen compositions of the present disclosure are also provided.
1. An engineered collagen composition prepared by the process of:
polymerizing a solution of oligomeric collagen to form a mixture of solid collagen and fluid;
confining the mixture of solid collagen and fluid; and
compressing the mixture of solid collagen and fluid to form an engineered collagen composition.
2. The engineered collagen composition of claim 1 , wherein the solution is non-acidic.
3. The engineered collagen composition of claim 1 , wherein the compression of the mixture of solid collagen and fluid removes fluid from the mixture.
4. An engineered collagen composition prepared by the process of:
polymerizing a solution of oligomeric collagen to form a collagen-fibril construct;
confining the collagen-fibril construct; and
compressing the collagen-fibril construct to form an engineered collagen composition.
5. The engineered collagen composition of claim 4 , wherein the solution is non-acidic.
6. The engineered collagen composition of claim 4 , wherein the compression removes fluid from the collagen-fibril construct.
7. The engineered collagen composition of claim 4 , wherein the confinement is in a container with one porous platen boundary.
8. The engineered collagen composition of claim 4 , wherein the confinement is in a container with more than one porous platen boundary.
9. The engineered collagen composition of claim 4 , wherein the engineered collagen composition possesses a gradient in at least one physical property.
10. The engineered collagen composition of claim 9 , wherein the property is density.
11. The engineered collagen composition of claim 4 , wherein the average density of collagen in the engineered collagen composition is greater than 3.5 mg/mL.
12. The engineered collagen composition of claim 4 , wherein the percentage of fluid which remains after compression is between 25% and 99% of the original fluid composition.
13. The engineered composition of claim 12 , wherein the percentage is between 50% and 80%.
14. The engineered composition of claim 4 in lyophilized form.
15. The engineered composition of claim 14 , wherein the lyophilized form is rehydrated.
16. The engineered composition of claim 4 , wherein the volume fraction of the fibrils in the engineered collagen composition is between about 1% to about 60%.
17. The engineered collagen composition of claim 4 , wherein the elastic modulus is between 0.4 MPa and 1.26 MPa.
18. The engineered collagen composition of claim 4 , wherein the compressive modulus is between about 17.0 kPa and about 178 kPa.
19. The engineered collagen composition of claim 4 , wherein the wet elastic modulus is between about 200 kP and 1.2 MPa.
20. The composition of claim 4 , wherein the compression is radial.
21. The engineered collagen composition of claim 4 , wherein compression is done by vacuum aspiration.
22. The engineered collagen composition of claim 11 , wherein the confinement is in a defined shape.
23. The engineered collagen composition of claim 12 , wherein the defined shape is a cylinder.
24. The engineered collagen composition of claim 13 , wherein the defined shape is selected from a tube, sheet, sphere, a slab, and a cone.
25. The engineered collagen composition of claim 4 , wherein the elastic modulus is greater than 0.4 MPa.
26. The engineered collagen composition of claim 4 , wherein the average density of collagen in the engineered collagen composition is between about 3 mg/ml and about 30 mg/ml.
27. The engineered collagen composition of claim 26 , wherein the average density of collagen in the engineered collagen composition is about 3.5 mg/ml.
28. The engineered collagen composition of claim 26 , wherein the average density of collagen in the engineered collagen composition is between about 4 mg/ml and about 10 mg/ml.
29. The engineered collagen composition of claim 26 , wherein the average density of collagen in the engineered collagen composition is between about 10 mg/ml and about 20 mg/ml.
30. The engineered collagen composition of claim 26 , wherein the average density of collagen in the engineered collagen composition is between about 20 mg/ml and about 30 mg/ml.