Microscope, focusing unit, fluid holding unit, and optical unit
A microscope includes: a sample placement part having a placement surface on which to place a sample and a bottom face opposite to the placement surface; an observation lens; and an optical unit for generating sheet light and use of the microscope. The microscope of an embodiment is arranged such that the sheet light enters the sample placement part from the bottom face and passing through the sample placement part to irradiate the sample, and fluorescence from the sample passes through the sample placement part toward the bottom face to be received by the observation lens. The microscope can, with this arrangement, utilize the advantages of a SPIM, and allows observation of a sample which observation is free from a restriction of the size of a sample.
1. A microscope, comprising:
a sample placement part having a placement surface on which to place a sample and a bottom face opposite to the placement surface;
an observation lens, arranged below the placement surface and the bottom face, for receiving fluorescence from the sample; and
an optical unit, arranged below the placement surface and the bottom face, for generating sheet light traveling in a direction parallel to an observation plane of the observation lens,
wherein
the optical unit is provided outside of the observation lens.
2. The microscope according to claim 1 , further comprising
a focusing mechanism for adjusting a relative positional relationship between the observation lens and the sample,
wherein
the focusing mechanism is configured to change the positional relationship along a first direction, which is parallel to an optical axis of the observation lens, and a second direction and a third direction, which define the placement surface.
3. The microscope according to claim 2 , further comprising
a fluid holding device for holding fluid between the observation lens and the sample placement part,
wherein:
the fluid holding device comprises:
a connection section for connection to the observation lens;
an end face so designed as to face the bottom face;
a transmission window through which the sheet light may enter the sample placement part; and
a fluid holding section for holding fluid inside itself; and
when the fluid holding device is connected to the observation lens, the end face is substantially parallel to the bottom face, the sheet light may pass through the transmission window and then through the fluid holding section to irradiate the sample, and the fluorescence passes through the fluid holding section to be received by the observation lens.
4. The microscope according to claim 3 ,
wherein:
the observation lens is an immersion lens; and
the fluid holding section is filled with a liquid corresponding to the immersion lens.
5. The microscope according to claim 4 ,
wherein:
the fluid holding section is filled with water; and
the sample placement part has a refractive index of 1.28 to 1.38.
6. The microscope according to claim 2 ,
wherein
the observation plane and the placement surface form an angle within a range from 1 degree to 75 degrees.
7. The microscope according to claim 2 ,
wherein:
the optical unit comprises an optical surface plate having a surface on which one or more optical elements for generating the sheet light are disposed; and
the optical surface plate is oriented so that the surface of the optical surface plate is parallel to the observation plane.
8. The microscope according to claim 7 ,
wherein:
the one or more optical elements comprise a light source, a light converging element, and a light distributing element; and
the one or more optical elements optionally further comprise a wedge prism.
9. The microscope according to claim 1 , further comprising
a fluid holding device for holding fluid between the observation lens and the sample placement part,
wherein:
the fluid holding device comprises:
a connection section for connection to the observation lens;
an end face so designed as to face the bottom face;
a transmission window through which the sheet light may enter the sample placement part; and
a fluid holding section for holding fluid inside itself; and
when the fluid holding device is connected to the observation lens, the end face is substantially parallel to the bottom face, the sheet light may pass through the transmission window and then through the fluid holding section to irradiate the sample, and the fluorescence may pass through the fluid holding section to be received by the observation lens.
10. The microscope according to claim 9 ,
wherein:
the observation lens is an immersion lens; and
the fluid holding section is filled with a liquid corresponding to the immersion lens.
11. The microscope according to claim 10 ,
wherein:
the fluid holding section is filled with water; and
the sample placement part has a refractive index of 1.28 to 1.38.
12. The microscope according to claim 1 ,
wherein
the observation plane and the placement surface form an angle within a range from 1 degree to 75 degrees.
13. The microscope according to claim 1 ,
wherein:
the optical unit comprises an optical surface plate having a surface on which one or more optical elements for generating the sheet light are disposed; and
the optical surface plate is oriented so that the surface of the optical surface plate is parallel to the observation plane.
14. The microscope according to claim 13 ,
wherein:
the one or more optical elements comprise a light source, a light converging element, and a light distributing element; and
the one or more optical elements optionally further comprise a wedge prism.
15. A focusing device for, in a case where a sample on a placement surface of a sample placement part is to be observed, adjusting a relative positional relationship between an observation lens having an optical axis not orthogonal to the placement surface and the sample,
the focusing device comprising:
a first stage for changing the relative positional relationship between the observation lens and the sample along a first direction, which is parallel to the optical axis of the observation lens;
a second stage for changing the relative positional relationship between the observation lens and the sample along a second direction and a third direction, which define the placement surface; and
a jig for fixing the sample placement part,
the jig being attached to one of the first stage and the second stage,
the stage to which the jig is attached being placed on the other stage to which the jig is not attached.
16. A fluid holding device for holding fluid between an observation lens and a sample placement part having a placement surface on which to place a sample to be observed and a bottom face opposite to the placement surface,
the fluid holding device comprising:
a connection section for connection to the observation lens;
an end face so designed as to face the bottom face;
a transmission window through which sheet light may enter the sample placement part; and
a fluid holding section for holding fluid inside itself,
when the fluid holding device is connected to the observation lens, the end face being substantially parallel to the bottom face, the sheet light from an optical unit may pass through the transmission window and then through the fluid holding section to irradiate the sample, and fluorescence from the sample passing through the fluid holding section to be received by the observation lens.
17. A microscope, comprising:
a sample placement part having a placement surface on which to place a sample and a bottom face opposite to the placement surface;
an observation lens for receiving fluorescence from the sample; and
an optical unit for generating sheet light having a thickness along a direction parallel to an optical axis of the observation lens,
the optical unit being provided outside of the observation lens, wherein the optical unit is arranged such that sheet light enters the sample placement part from the bottom face and passes through the sample placement part to irradiate the sample, and the fluorescence passes through the sample placement part toward the bottom face to be received by the observation lens.
18. A microscope, comprising:
a sample placement part having a placement surface on which to place a sample and a bottom face opposite to the placement surface;
an observation lens for receiving fluorescence from the sample; and
an optical unit for irradiating the sample with sheet light in a direction perpendicular to an optical axis of the observation lens,
the optical unit being provided outside of the observation lens, wherein the optical unit is arranged such that sheet light entering the sample placement part from the bottom face and passes through the sample placement part to irradiate the sample in the direction perpendicular to the optical axis of the observation lens, and the fluorescence passes through the sample placement part toward the bottom face to be received by the observation lens.