IP Library › Granted Patent US 9,890,409
Granted Patent B2
US 9,890,409 · App. 15/145,679 · Granted Feb 13, 2018

Composition for astringin product

Inventors: Chul-Ho Yun (Daejeon, KR); Hyung-Sik Kang (Gwangju, KR); Young-Hee Joung (Daejeon, KR); Gun Su Cha (Gwangju, KR)
Assignee: Industry Foundation of Chonnam National University
C12P19/46C12N9/0071C12Y114/14001
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Quick Facts
Patent No.
US 9,890,409
App. No.
15/145,679
Granted
Feb 13, 2018
Kind
B2
Abstract

Provided is a composition for producing astringin among metabolites of polydatin, wherein the astringin may be mass-produced by oxidizing the polydatin using a CYP102A1 chimera and mutants thereof as a catalyst, the CYP102A1 chimera being produced by fusing a reductase domain of a wild-type CYP102A1 which is a bacterial cytochrome P450 enzyme, with a heme domain of a CYP102A1 mutant.

Claims (5)

1. A composition for producing astringin from polydatin comprising a mutant of a CYP102A1 chimera,

wherein the mutant of the CYP102A1 chimera is F11L/F81I/Q110P/R190Q of the CYP102A1 chimera, and

wherein the CYP102A1 chimera consists of the amino acid sequence of SEQ ID NO: 18.

2. A kit for producing astringin from polydatin comprising a mutant of a CYP102A1 chimera and an NADPH-generating system,

wherein the mutant of the CYP102A1 chimera is F11L/F81I/Q110P/R190Q of the CYP102A1 chimera; and wherein the CYP120A1 chimera consists of the amino acid sequence of SEQ ID NO: 18.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 4, 2016
From: YUN, CHUL-HO; KANG, HYUNG-SIK; JOUNG, YOUNG-HEE; CHA, GUN SU
To: INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY
Reel/Frame 038457/0449 →
Priority Claims (1)
KR 10-2015-0128399 · Sep 10, 2015 · national
Continuity (1)
Related Publication 20170073720A1 · Mar 16, 2017