Method and apparatus for tissue grafting and copying
Exemplary embodiments of apparatus and method for obtaining one or more portions of biological tissue (“micrografts”) to form grafts are provided. For example, a hollow tube can be inserted into tissue at a donor site, and a pin provided within the tube can facilitate controlled removal of the micrograft from the tube. Micrografts can be harvested and directly implanted into an overlying biocompatible matrix through coordinated motion of the tube and pin. A needle can be provided around the tube to facilitate a direct implantation of a micrograft into a remote recipient site or matrix. The exemplary apparatus can include a plurality of such tubes and pins for simultaneous harvesting and/or implanting of a plurality of micrografts. The harvested micrografts can have a small dimension, e.g., less than about 1 mm, which can promote healing of the donor site and/or viability of the harvested tissue.
1. A method for obtaining at least one portion of at least one biological tissue, comprising:
providing a matrix material over a donor tissue site;
positioning a distal end of at least one hollow tube proximal to the upper surface of the matrix material;
positioning a pin within a lumen of the at least one tube such that the distal end of the pin is proximal to the distal end of the at least one tube;
lowering the pin and the at least one tube together substantially through a thickness of the matrix material, such that the distal ends of the at least one tube and the pin are proximal to the surface of the donor tissue site;
advancing the at least one tube into the donor site to sever the at least one portion of the at least one biological tissue from a surrounding tissue, wherein the distal end of the pin remains proximal to the surface of the donor tissue site;
raising the at least one tube and the pin simultaneously at substantially the same rate until the distal end of the at least one tube is proximal to a lower surface of the matrix material; and
retracting the at least one tube from the matrix while maintaining a location of the pin substantially stationary relative to the matrix material, such that the at least one portion of the at least one biological tissue remains within the matrix material.
2. The method of claim 1 , further comprising providing at least one growth factor in the matrix material.
3. The method of claim 1 , wherein the matrix material comprises at least one of collagen, low-melting agarose, or devitalized biological tissue.