Non-thermal cycling for polymerase chain reaction
View Patent ↗Techniques, systems, and devices are disclosed for non-thermal cycling of polymerase chain reaction (PCR). In one aspect, a method for cycling PCR includes receiving an electrolytic fluid including ions, primers, polymerase enzymes, nucleotides, and a double-stranded nucleic acid in a fluid chamber having a first electrode and a second electrode, applying an electric field across the first and the second electrodes to generate a first pH level of the electrolytic fluid to denature the double-stranded nucleic acid to at least partial single strands, and applying a second electric field across the first and second electrodes to produce a second pH level of the electrolytic fluid, in which the second pH level enables binding of a polymerase enzyme and a primer with a corresponding segment of the single strands.
1. A device for cycling polymerase chain reaction (PCR), comprising:
a container including an interior surface formed of an electrically insulative material and structured to contain an electrolytic fluid including ions, primers, polymerase enzymes, nucleotides, and a double-stranded nucleic acid;
a first electrode and a second electrode configured on an interior surface of the container in contact with the electrolytic fluid; and
a circuit electrically coupled to the first and second electrodes to apply a first electric field across the first and second electrode to generate a first pH level of the electrolytic fluid, wherein the double-stranded nucleic acid is capable of denaturation to at least partial single strands at the first pH level, and to apply a second electric field across the first and second electrodes to generate a second pH level of the electrolytic fluid, wherein the second pH level enables at least one of binding of a polymerase enzyme and a primer with a corresponding segment of the at least partial single strands or synthesis of new complementary nucleic acid strands from the nucleotides using the at least partial single strands.
2. The device as in claim 1 , further comprising a semi-permeable membrane configured within the interior of the container and structured to separate an interior of the container into a first region including the first electrode and a second region including the second electrode, the semi-permeable membrane permeable for the ions to traverse the membrane and impermeable to the primers, polymerase enzymes, nucleotides, and double-stranded nucleic acid.
3. The device as in claim 2 , wherein the semi-permeable membrane is moveable within the container to adjust a volume of the first or the second region.
4. The device as in claim 1 , wherein the polymerase enzymes are covalently attached to a surface of the fluid chamber.