Hand-held measuring instrument and procedure for the detection of mold attack in interior spaces
A hand-held measuring instrument is used for detecting hidden mold damage in interior spaces. The measuring instrument includes at least one collection unit configured to intake at least of one interior space sample, at least one buffer unit, at least one immunological test unit, an evaluation unit and a control panel. The at least one buffer unit has a first compartment and a second compartment.
1. A method for detecting hidden mold damage in interior spaces using a hand-held measuring instrument, the method comprising:
collecting an interior space sample;
taking up the sample in a buffer;
concentrating at least one analyte in the sample taken up in the buffer by binding the at least one analyte to analyte-specific antibodies in a first compartment of the hand-held measuring instrument so as to form antibody-analyte complexes;
transferring the antibody-analyte complexes to a second compartment of the hand-held measuring instrument, wherein the second compartment comprises immobilized secondary antibodies capable of binding the analyte-specific antibodies so as to immobilize the antibody-analyte complexes via the immobilized secondary antibodies, and wherein the at least one analyte is at least one mold spore;
removing unbound constituents;
eluting the analytes from the analyte-specific antibodies;
detecting the eluted analytes using an immunological test within the hand-held measuring instrument;
evaluating the immunological test; and
displaying a result of the evaluated test, thereby detecting hidden mold damage in the interior space.
2. The method of claim 1 , wherein the analyte-specific antibodies are directed against surface markers of mold spores.
3. The method of claim 2 , wherein the surface markers are β-glucans.
4. The method of claim 1 , wherein the analyte-specific antibodies are species-specific antibodies to mold spores.
5. The method of claim 1 , wherein the result of the evaluated test corresponds to one of: (i) a total signal for a number of mold spores contained in the sample and (ii) a species-specific signal of a mold species.
6. The method of claim 1 , wherein the result of the evaluated test corresponds to a total signal for a number of mold spores contained in the sample.
7. The method of claim 1 , wherein the secondary antibodies are immobilized on a solid phase of the second compartment.