Injectable silk fibroin particles and uses thereof
The inventions provided herein relate to compositions, methods, delivery devices and kits for repairing or augmenting a tissue in a subject. The compositions described herein are injectable such that they can be placed in a tissue to be treated with a minimally-invasive procedure (e.g., by injection) and/or be molded flexibly into a tissue void of any shape and/or size. In some embodiments, the composition described herein comprises a plurality of silk fibroin particles, which can retain their original volume within the tissue for a period of time. The compositions can be used as a filler to replace a tissue void, e.g., for tissue repair and/or augmentation, or as a scaffold to support tissue regeneration and/or reconstruction. In some embodiments, the compositions described herein can be used for soft tissue repair or augmentation.
1. An injectable composition comprising:
porous silk fibroin particles in an injectable format, wherein the porous silk fibroin particles have a porosity of at least 70, and wherein the silk fibroin constitutes at least 30% of the total composition; and
wherein the injectable composition further comprises a biological fluid comprising lipoaspirate in a volume ratio between 3:19 to 6:19 silk fibroin to lipoaspirate,
wherein the processed lipoaspirate has not been subjected to preferential expansion of one cell type over another.
2. The injectable composition of claim 1 , wherein the tissue site is a soft tissue site.
3. The injectable composition of claim 1 , wherein the tissue site is a hard tissue site.
4. The injectable composition of claim 3 , wherein the hard tissue site is bone tissue.
5. The injectable composition of claim 1 , wherein the porous silk fibroin particles have a size of about 500 μm to about 5,000 μm.
6. The injectable composition of claim 2 , wherein the porous silk fibroin particles retain at least about 50% of their original volume after being placed into the soft tissue site in the subject for the period of time.
7. The injectable composition of claim 1 , wherein the period of time is at least about 3 months.
8. The injectable composition of claim 1 , wherein the period of time is at least about 6 months.
9. The injectable composition of claim 1 , wherein the porous silk fibroin particles have a porosity of at least about 90.
10. A delivery device comprising the injectable composition of claim 1 .
11. The delivery device of claim 10 , further comprising an injection carrier.
12. A method of repairing or augmenting a tissue site in a subject comprising administering the injectable composition of claim 1 .
13. The composition of claim 1 , further comprising bone-marrow aspirate.
14. The composition of claim 13 , wherein the bone-marrow aspirate is in a volume ratio of about 1:38 to 12:19 silk fibroin to biological fluid or concentrate.
15. The composition of claim 1 , wherein the lipoaspirate comprises one or more cells.
16. The composition of claim 15 , wherein the one or more cells is not cultured prior to administration.