Prevention of
The present invention provides an agent for controlling Escherichia coli diarrhea comprising a Shiga toxin as an active ingredient.
1. A method for controlling Escherichia coli diarrhea. in a pig infected with the Escherichia coli , the method comprising administering a Shiga toxin or a transformant containing the Shiga toxin to the pig in need thereof, wherein the Shiga toxin is a hybrid protein comprising two Shiga toxin B subunit proteins (Stx2eB) tandemly linked via a peptide linker, and wherein the Escherichia coli produces heat-labile toxin (LT) and/or heat-stable toxin (ST) but does not produce Stx2e toxin.
2. The method according to claim 1 , wherein the Shiga toxin is administered as a transformant which is transformed with a recombinant vector containing a DNA construct comprising a DNA coding for the hybrid protein, and expresses the hybrid protein.
3. The method according to claim 1 , wherein the hybrid protein is administered as a transformant which is transformed with a recombinant vector containing a DNA construct comprising a DNA coding for the hybrid protein, and expresses the hybrid protein.
4. The method according to claim 2 , wherein the transformant is a vegetative-propagation plant.
5. The method according to claim 4 , wherein the vegetative-propagation plant s strawberry.
6. The method according to claim 1 , wherein the pig is a pig in the lactation period or of up to 120-day old, or a mother pig.
7. A method of improving fattening of a pig infected with a toxin-producing Escherichia coli , comprising administering a Shiga toxin as an active ingredient to the pig in need thereof, wherein said Shiga toxin protein is a hybrid protein comprising two Shiga toxin B subunit proteins (Stx2eB) tandemly linked via a peptide linker, and wherein said Escherichia coli produces heat-labile toxin (LT) and/or heat-stable toxin (ST) but does not produce Stx2e toxin.
8. The method according to claim 7 , wherein the Shiga toxin is administered as a transformant which is transformed with a recombinant vector containing a DNA construct comprising a DNA coding for the hybrid protein, and expresses the hybrid protein.
9. The method according to claim 7 , wherein the Shiga toxin is administered as a transformant which is transformed with a recombinant vector containing a DNA construct comprising a DNA coding for a hybrid protein comprising the hybrid protein, and expresses the hybrid protein.
10. The method according to claim 8 , wherein the transformant is a vegetative-propagation plant.
11. The method according to claim 10 , wherein the vegetative-propagation plant is strawberry.