Beta sheet tapes ribbons in tissue engineering
There is described a material comprising tapes, ribbons, fibrils or fibers characterized in that each of the ribbons, fibrils or fibers have an antiparallel arrangement of peptides in a β-sheet tape-like substructure.
1. A self assembling polypeptide (SAP) that forms ribbons, fibrils or fibres in a β-sheet tape-like substructure at physiological pH and physiological salt concentrations, and wherein the polypeptide consists of P 11-20 (QQRQEQEQEQQ, SEQ ID NO: 12), wherein an N-terminus of the peptide is blocked with CH 3 CO- and a C-terminus of the peptide is blocked with NH 2 —.
2. A dental product comprising the self-assembling polypeptide of claim 1 .
3. A dental repair or reconstruction or preventative product comprising the self-assembling polypeptide of claim 1 .
4. A tissue engineering scaffold comprising the self-assembling polypeptide of claim 1 .
5. The tissue engineering scaffold according to claim 4 , wherein the scaffold is seeded with cells.
6. A bone repair or reconstruction product comprising the self-assembling polypeptide of claim 1 .
7. The bone repair or reconstruction product according to claim 6 , wherein the product is seeded with cells.
8. A soft tissue or reconstruction product comprising the self-assembling polypeptide of claim 1 .
9. A skin treatment comprising the self-assembling polypeptide of claim 1 .
10. A hair care product comprising the self-assembling polypeptide of claim 1 .
11. A bioresponsive and biocompatible surface comprising the self-assembling polypeptide of claim 1 .
12. The self-assembling polypeptide of claim 1 , comprising continuous filaments, yarns, staple, floc, cord, or fabric.
13. The self-assembling polypeptide of claim 1 , further comprising a polymer.
14. The self-assembling polypeptide of claim 1 , wherein the physiological pH is about 7.5.
15. The self-assembling polypeptide of claim 1 , wherein the physiological salt concentration is about 145 mM NaCl.
16. A composition, comprising:
ribbons, fibrils or fibres, wherein each of the ribbons, fibrils or fibres has an antiparallel arrangement of peptides in a β-sheet tape-like substructure at physiological pH and physiological salt concentrations, wherein the composition comprises
a peptide consisting of P 11-20 (QQRQEQEQEQQ, SEQ ID NO: 12); and
a physiological concentration of salt.
17. The composition of claim 16 , wherein the composition comprises fibrils, and wherein the fibrils are comprised in a network of fibrils interconnected at fibre-like junctions.
18. A dental composition comprising the fibrils of claim 16 .
19. A dental repair or reconstruction or preventative composition comprising the fibrils of claim 16 .
20. The composition of claim 16 , wherein the composition is in the form of a tissue engineering scaffold.
21. The composition according to claim 20 , comprising the scaffold seeded with cells.
22. A bone repair or reconstruction composition comprising the fibrils of claim 16 .
23. A soft tissue or reconstruction composition comprising the fibrils of claim 16 .
24. The composition according to claim 22 that is seeded with cells.
25. A skin treatment comprising the composition of fibrils of claim 16 .
26. A hair care product comprising the composition of fibrils of claim 16 .
27. A part of a bioresponsive and biocompatible surface comprising the composition of fibrils of claim 16 .
28. The composition of claim 16 , wherein the composition comprises continuous filament yarns, staple, floc, cord, or fabric.
29. The composition of claim 16 , further comprising a polymer.
30. The composition of claim 16 , wherein the physiological pH is about 7.5.
31. The composition of claim 16 , wherein the physiological salt concentration is about 145 mM NaCl.
32. A method of forming a tape, comprising contacting the SAP of claim 1 with an ionic aqueous medium, thereby forming a tape.
33. A method of forming a gel, comprising contacting the SAP of claim 1 with an ionic aqueous medium, thereby forming a gel.
34. A method of forming a tape, comprising contacting the composition of claim 16 with an ionic aqueous medium, thereby forming a tape.
35. A method of forming a gel, comprising contacting the composition of claim 16 with an ionic aqueous medium, thereby forming a gel.
36. A composition comprising:
a self assembling polypeptide (SAP) consisting of P 11-20 (QQRQEQEQEQQ, SEQ ID NO: 12), and
an aqueous solution at a physiological pH and comprising a physiological concentration of salt,
wherein the peptide forms ribbons, fibrils or fibres in a β-sheet tape-like substructure at the physiological pH and at the physiological concentration of salt.
37. The composition of claim 36 , wherein the physiological pH is pH 7 to pH 8, and the physiological concentration of salt is 120 mM to 160 mM NaCl.
38. The composition of claim 36 , wherein the physiological pH is pH 7.5 and the physiological concentration of salt is about 145 mM NaCl.
39. A composition comprising:
a self assembling polypeptide (SAP) consisting of P 11-20 (QQRQEQEQEQQ, SEQ ID NO: 12), and
a cotton or wool fiber.
40. A tissue engineering scaffold, comprising;
a self assembling polypeptide (SAP) consisting of P 11-20 (QQRQEQEQEQQ, SEQ ID NO: 12), and
ligamentum cells, tenocytes, chondrocytes and/or chondrocyte progenitor cells.