Peronospora resistance in
The present invention relates to a spinach plant which may comprise an allele conferring resistance to downy mildew, propagation material of said spinach plant, a cell of said spinach plant, seed of said spinach plant, and to harvested leaves of said spinach plant. The invention further relates to use of a spinach plant in breeding to confer resistance against downy mildew.
1. An agronomically elite Spinacia oleracea spinach plant comprising a single dominant resistance allele that confers resistance to at least Peronospora farinosa f. sp. spinaciae races Pfs:1, Pfs:2, Pfs:3, Pfs:4, Pfs:5, Pfs:6, Pfs:9, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15 and isolate UA1014 and not resistance to Peronospora farinosa f. sp. spinaciae race Pfs:7, whereby the agronomically elite Spinacia oleracea plant is resistant to at least Peronospora farinosa f. sp. spinaciae races Pfs: 1, Pfs:2, Pfs:3, Pfs:4, Pfs:5, Pfs:6, Pfs:9, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15 and isolate UA1014 and not resistance to Peronospora farinosa f. sp. spinaciae race Pfs:7, wherein said allele is linked to
a SNP of C at nucleotide 33 of SEQ ID NO: 1,
a SNP of T at nucleotide 79 of SEQ ID NO: 5,
a SNP of T at nucleotide 79 of SEQ ID NO: 7, and
an indel of SEQ ID NO: 3 comprising deletion of CGAT in SEQ ID NO:4;
and wherein said allele is present in seed deposited with the NCIMB under NCIMB accession number 42466.
2. The agronomically elite Spinacia oleracea plant of claim 1 comprising the allele heterozygously, wherein the heterozygous presence of said allele further confers at least intermediate resistance to Peronospora farinosa f sp. spinaciae races Pfs:8 and Pfs:10.
3. The agronomically elite Spinacia oleracea plant of claim 1 comprising the allele homozygously, wherein the homozygous presence of said allele further confers resistance to Peronospora farinosa f. sp. spinaciae race Pfs:8 and intermediate resistance to Peronospora farinosa f. sp. spinaciae race Pfs:10.
4. The agronomically elite Spinacia oleracea plant of claim 1 , wherein said plant is a plant of an inbred line or a hybrid plant.
5. The agronomically elite Spinacia oleracea plant of claim 1 , wherein the spinach plant is further resistant to Peronospora farinosa f. sp. spinaciae race Pfs:7.
6. A Spinacia oleracea propagation material comprising:
a single dominant resistance allele that confers resistance to at least Peronospora farinosa f. sp. spinaciae races Pfs:1, Pfs:2, Pfs:3, Pfs:4, Pfs:5, Pfs:6, Pfs:9, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15 and isolate UA1014 and not resistance to Peronospora farinosa f. sp. spinaciae race Pfs:7,
wherein said allele is
linked to:
a SNP of C at nucleotide 33 of SEQ ID NO: 1,
a SNP of T at nucleotide 79 of SEQ ID NO: 5,
a SNP of T at nucleotide 79 of SEQ ID NO: 7, and
an indel of SEQ ID NO: 3 comprising deletion of CGAT in SEQ ID NO: 4, and
wherein the propagation material comprises:
a microspore,
a pollen,
an ovary,
an ovule,
an embryo,
an embryo sac,
an egg cell,
a cutting,
a root,
a root tip,
a hypocotyl,
a cotyledon,
a stem,
a leaf,
a flower,
an anther,
a seed,
a meristematic cell,
a protoplast,
a cell,
or a tissue culture,
of an agronomically elite Spinacia oleracea plant,
and wherein said allele is present in seed deposited with the NCIMB under NCIMB accession number 42466.
7. A Spinacia oleracea cell comprising:
a single dominant resistance allele that confers resistance to at least Peronospora farinosa f. sp. spinaciae races Pfs:1, Pfs:2, Pfs:3, Pfs:4, Pfs:5, Pfs:6, Pfs:9, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15 and isolate UA1014 and not resistance to Peronospora farinosa f. sp. spinaciae race Pfs:7,
wherein said allele is
linked to:
a SNP of C at nucleotide 33 of SEQ ID NO: 1,
a SNP of T at nucleotide 79 of SEQ ID NO: 5,
a SNP of T at nucleotide 79 of SEQ ID NO: 7, and
an indel of SEQ ID NO: 3 comprising deletion of CGAT in SEQ ID NO:4 and,
and wherein said allele is present in seed deposited with the NCIMB under NCIMB accession number 42466; and
wherein the cell is of an agronomically elite Spinacia oleracea plant.
8. An agronomically elite Spinacia oleracea seed comprising:
a single dominant resistance allele that confers resistance to at least Peronospora farinosa f. sp. spinaciae races Pfs:1, Pfs:2, Pfs:3, Pfs:4, Pfs:5, Pfs:6, Pfs:9, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15 and isolate UA1014 and not resistance to Peronospora farinosa f. sp. spinaciae race Pfs:7,
wherein said allele is
linked to:
a SNP of C at nucleotide 33 of SEQ ID NO: 1,
a SNP of T at nucleotide 79 of SEQ ID NO: 5,
a SNP of T at nucleotide 79 of SEQ ID NO: 7, and
an indel of SEQ ID NO: 3 comprising deletion of CGAT in SEQ ID NO:4 and,
and wherein said allele is present in seed deposited with the NCIMB under NCIMB accession number 42466; and
wherein the seed gives rise to an agronomically elite Spinacia oleracea plant.
9. One or more harvested leaves of the agronomically elite Spinacia oleracea plant of claim 1 .
10. A food product comprising the one or more harvested leaves of claim 9 .
11. A container comprising the one or more harvested leaves of claim 9 .
12. A container comprising one or more agronomically elite Spinacia oleracea plants of claim 1 .
13. The container of claim 12 wherein the one or more spinach plants are in a growth substrate for harvest of leaves from the one or more spinach plants.