Medium for the protein-free and serum-free cultivation of cells
A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.
1. A method for increasing productivity of a recombinant protein in a cell culture, the method comprising:
culturing mammalian cells that express the recombinant protein in an animal protein-free and serum-free cell culture medium; and
adding more than 10 wt. % ultrafiltered soy hydrolysate based on the total dry weight of the medium to the animal protein-free and serum-free cell culture medium,
wherein at least 40% of the soy hydrolysate has a molecular weight of ≤500 daltons.
2. The method of claim 1 , wherein at least 50% of the soy hydrolysate has a molecular weight of ≤500 daltons.
3. The method of claim 1 , wherein at least 55% of the soy hydrolysate has a molecular weight of ≤500 daltons.
4. The method of claim 1 , wherein the soy hydrolysate has an endotoxin content of <500 U/g.
5. The method of claim 1 , wherein the mammalian cells are CHO cells or BHK cells.
6. The method of claim 1 , wherein the recombinant protein is selected from the group consisting of: Factor II, Factor V, Factor VII, Factor VIII, Factor IX, Factor X, Factor XI, Protein S, Protein C, activated forms of these factors, and vWF.
7. The method of claim 6 , wherein the recombinant protein is Factor VIII.
8. The method of claim 1 , wherein the medium comprises an amino acid.
9. The method of claim 8 , wherein the amino acid is selected from the group consisting of L-asparagine, L-cysteine, L-cystine, L-proline, L-tryptophan, L-glutamine and mixtures thereof.
10. The method of claim 1 , wherein the medium further comprises: 1 to 100 g/L synthetic minimal medium; 0.05-1 g/L glutamine; 0.0005-0.05 g/L ascorbic acid; 0.1-10 g/L NaHCO 3 ; 0.0005-0.05 g/L ethanolamine; and 1-15 μg/L sodium selenite.
11. The method of claim 1 , wherein the cells are cultured in a bioreactor.