Microscopy support structures
Electron microscope support structures and methods of making and using same. The support structures are generally constructed using semiconductor materials and semiconductor manufacturing processes. The temperature of the support structure may be controlled and/or gases or liquids may be confined in the observation region for reactions and/or imaging.
1. An environmental cell comprising a holder, a window device, and a temperature control device, wherein the window device and the temperature control device are stacked in a columnar arrangement in the holder and form an observation region for confining gas or liquid; wherein:
the temperature control device comprises a first membrane comprising a top side, a bottom side, and at least one membrane observation region;
the temperature control device comprises at least two conductive heat source elements in contact with the top side of the first membrane forming a heatable region of the first membrane; and
the at least two conductive heat source elements flank the at least one membrane observation region.
2. The environmental cell of claim 1 , wherein the first membrane comprises a semiconductor material selected from the group consisting of silicon nitride, silicon carbide, boron nitride, graphene, carbon, aluminum nitride, silicon dioxide, silicon and combinations thereof.
3. The environmental cell of claim 1 , wherein at least one of the at least two conductive heat source elements comprises a semiconductor material that is more conductive than the first membrane.
4. The environmental cell of claim 1 , wherein the at least two conductive heat source elements comprise a material selected from the group consisting of silicon carbide, aluminum nitride, boron nitride, monocrystalline silicon, polycrystalline silicon, amorphous silicon, other semiconductor materials, metals, ceramics, conducting oxides, and combinations thereof.
5. The environmental cell of claim 1 , wherein the window device and the temperature control device are aligned to provide a path for an electron beam and enable electron beam imaging.
6. The environmental cell of claim 5 , configured so that the electron beam is first incident upon the window device before it is incident upon the temperature control device.
7. The environmental cell of claim 5 , configured so that the electron beam is first incident upon the temperature control device before it is incident upon the window device.
8. The environmental cell of claim 1 , wherein the window device comprises a second membrane, and wherein a void between the first membrane and the second membrane defines the at least one membrane observation region.
9. The environmental cell of claim 1 , wherein the window device and the temperature control device are at least one of mechanically and electrically mounted in the holder.
10. The environmental cell of claim 1 , wherein the at least one membrane observation region is heatable.
11. The environmental cell of claim 1 , wherein the at least two conductive heat source elements are arranged so that current can be forced through the first membrane thus allowing Joule heating to occur in the at least one membrane observation region.
12. The environmental cell of claim 1 , wherein the window device comprises a frame, and a second membrane.
13. The environmental cell of claim 1 , wherein the first membrane is supported by a frame, wherein the frame is located on the bottom side of the first membrane and positioned around a perimeter of the temperature control device.
14. The environmental cell of claim 1 , wherein the at least two conductive heat source elements comprise material patterned directly on the first membrane, wherein said material comprises a ceramic material having a conductivity greater than the conductivity of the first membrane.
15. The environmental cell of claim 1 , further comprising a spacer between the window device and temperature control device to define a distance between the devices.
16. The environmental cell of claim 15 , wherein the spacer comprises a soft material and simultaneously seals the window device and temperature control device.
17. The environmental cell of claim 15 , wherein the spacer is patterned directly on at least one surface.
18. A method of imaging a specimen using an environmental cell, the method comprising:
providing an environmental cell comprising:
a holder, a window device, and a temperature control device, wherein the window device and the temperature control device are stacked in a columnar arrangement in the holder and form an observation region for confining gas or liquid; wherein:
the temperature control device comprises a first membrane comprising a top side, a bottom side, and at least one membrane observation region;
the temperature control device comprises at least two conductive heat source elements in contact with the top side of the first membrane forming a heatable region of the membrane; and
the at least two conductive heat source elements flank the at least one membrane observation region;
positioning a specimen in the at least one membrane observation region;
introducing a gas or a liquid to the at least one membrane observation region;
controlling the temperature of the specimen; and
imaging the specimen using an electron beam.