IP Library Granted Patent US 9,986,750
Granted Patent B2
US 9,986,750 · App. 14/812,598 · Granted Jun 5, 2018

Animal feed compositions and feed additives

Inventors: Richard S. Carpenter (West Chester, OH); JoElla Barnes (Arcola, IL); Michael Stanford Showell (Cincinnati, OH)
Assignee: BiOWiSH Technologies Inc.
A23K1/009A23K10/18A23K10/30A23K40/20A23K40/25A23K40/30A61K9/1664A61K31/733A61K35/742A61K35/747A23Y2220/67A23Y2280/15A23Y2280/55
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Quick Facts
Patent No.
US 9,986,750
App. No.
14/812,598
Granted
Jun 5, 2018
Kind
B2
Abstract

The present invention relates to pre-, pro-, and postbiotic compositions and methods of use for improving the health and nutrition of commercial livestock and companion pets.

Claims (27)

1. An animal feed composition comprising a mixture of:

a. a prebiotic selected from the group comprising inulin, fructo-, gluco-, or galcto-oligosaccharides;

b. a probiotic bacteria comprising a mixture of Lactobacillus microorganisms produced by submerged liquid fermentation wherein the resulting organisms have been dried to a moisture content less than about 10% then ground to produce a powder having a mean particle size of less than about 800 microns;

c. a Bacillus probiotic bacteria produced by submerged liquid fermentation wherein the resulting organism has been dried to a moisture content less than about 10% then ground to produce a powder having a mean particle size of less than about 800 microns;

d. a postbiotic, wherein the postbiotic is dried liquid fermentation medium of the Lactobacillus microorganisms; and

e. a filler material.

2. The composition of claim 1 wherein the mixture of Lactobacillus comprises Pediococcus acidilactici, Pediococcus pentosaceus , and Lactobacillus plantarum.

3. The composition of claim 2 wherein the mixture of Lactobacillus is prepared by mixing the individual Lactobacillus species produced via submerged liquid fermentation, wherein each of the Lactobacillus bacteria are individually anaerobically fermented to produce an individual anaerobic fermentation, harvested, dried, and ground to produce a powder having a mean particle size of 295 microns, with 60% of the mixture in the size range between 175-900 microns.

4. The composition of claim 1 wherein the Bacillus is a Bacillus subtilis having the 16S nucleic acid sequence of SEQ ID NO:1.

5. The composition of claim 3 , wherein the postbiotic is prepared by:

a. collecting the supernatants from the centrifugation of each of the individual anaerobic fermentations of Pediococcus acidilactici, Pediococcus pentosaceus , and Lactobacillus plantarum;

b. combing the supernatants obtained from step (a);

c. freeze-drying the mixed supernatants to a moisture content less than about 5% by weight; and

d. grinding the dried, mixed supernatants to a mean particle size of 295 microns, with 60% of the mixture in the size range between 175-900 microns.

6. The composition of claim 1 , wherein the filler comprises rice, soy, or wheat bran, rice, soy, or wheat flour, corn meal, rye, barley, sorghum, dextrose, sucrose, fructose, maltodextrin, starch or any combination thereof.

7. The composition of claim 1 , wherein the prebiotic, lactobacillus probiotic, and postbiotic components are combined in equal weight proportion.

8. The composition of claim 7 , wherein the bacillus probiotic is mixed with the prebiotic, lactobacillus probiotic, and postbiotic mix at a ratio of from 1:1 to 1:20.

9. The composition of claim 1 , wherein the ratio of pre-, pro-, and postbiotic components to cereal grain filler ranges from about 1:1 to about 3:1.

10. The composition of claim 1 , wherein the final bacterial concentration is between about 10 6 and 10 11 colony forming units (cfu) per gram.

11. An animal feed composition comprising 0.01 to 10.0% of the composition of claim 1 .

12. An animal feed composition according to claim 11 , wherein the pre-, pro-, postbiotic mixture is coated onto the feed particles using a wax, fat, sugar, oil or polysaccharide binder.

13. An animal feed composition according to claim 11 , wherein the pre-, pro-postbiotic mixture is integrated into a feed pellet.

14. An animal feed composition according to claim 11 , wherein the pre-, pro-, postbiotic mixture is integrated into a mash feed.

15. A process for dosing animals with the pre-, pro-, and postbiotic mixture of claim 1 , wherein the mixture is dissolved into the animals' drinking water supply.

16. A process according to claim 15 wherein the pre-, pro-, and postbiotic mixture is dosed into the water supply between about 0.1 and 1 kg per 1000 liters.

17. A method of feeding an animal, the method comprising administering to the animal the animal feed composition according to any one of claims 1 to 14 .

18. The method of claim 17 , wherein the animal is shrimp, fin fish, poultry, swine, ruminants or companion pets.

Assignments (3)
RELEASE OF SECURITY INTEREST Recorded Aug 8, 2022
From: BARGA, LLC, AS COLLATERAL AGENT
To: BIOWISH TECHNOLOGIES, INC., ASSIGNOR
Reel/Frame 060748/0136 →
SECURITY INTEREST Recorded May 15, 2020
From: BIOWISH TECHNOLOGIES INTERNATIONAL, INC. (A DELAWARE CORPORATION)
To: BARGA, LLC (A TEXAS LIMITED LIABILITY COMPANY)
Reel/Frame 052679/0764 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 11, 2015
From: CARPENTER, RICHARD S.; BARNES, JOELLA; SHOWELL, MICHAEL STANFORD
To: BIOWISH TECHNOLOGIES INC.
Reel/Frame 036301/0557 →
Continuity (3)
Provisional Application 62030460 · Jul 29, 2014
Provisional Application 62090756 · Dec 11, 2014
Related Publication 20160029666A1 · Feb 4, 2016