Psicose epimerase and psicose production method using same
The present invention provides a novel psicose epimerase derived from Flavonifractor plautii and capable of converting fructose to psicose. The novel psicose epimerase according to the present invention possesses an activity producing psicose by epimerizing the carbon-3 position of fructose, and has maximal activity for the conversion of fructose to psicose at a relatively high temperature and a pH less than or equal to neutral, has excellent thermal stability, and can mass-produce psicose from fructose in a high yield for a short amount of time. Therefore, the psicose epimerase according to the present invention is advantageous in the industrial production of psicose, and it is expected that the psicose produced thereby can be usefully utilized in the functional sugar industry and also as materials for health food, medicine, cosmetics, and the like using the psicose.
1. A method for producing psicose, the method comprising:
reacting fructose with a psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1.
2. The method for producing psicose of claim 1 , wherein the psicose epimerase is obtained from Flavonifractor plautii.
3. The method for producing psicose of claim 1 , wherein the composition further includes one or more kinds selected from the group consisting of manganese ions, nickel ions, and cobalt ions.
4. The method for producing psicose of claim 1 , wherein a temperature of the reaction is 55 to 67° C. and a pH of the reaction is 6.5 to 8.
5. The method for producing psicose of claim 1 , wherein the concentration of the fructose is 35 to 75% (w/w).
6. The method for producing psicose of claim 1 , wherein the psicose epimerase is immobilized in a carrier.