Method to identify disease resistant quantitative trait loci in soybean and compositions thereof
The present invention is in the field of plant breeding and genetics, particularly as it pertains to the genus, Glycine . More specifically, the invention relates to a method for screening soybean plants containing one or more quantitative trait loci for disease resistance, species of Glycine having such loci and methods for breeding for and screening of Glycine with such loci. The invention further relates to the use of exotic germplasm in a breeding program.
1. A method of producing a population of soybean plants or seeds, said method comprising:
genotyping a first population of soybean plants or seeds for the presence of a molecular marker that is within 10 centimorgans or less from SNP marker NS0124935, wherein said SNP marker NS0124935 is linked to Asian Soybean Rust (ASR) resistance locus 10, 11, 12, or 13 in haplotype window 2 on chromosome 13;
selecting from said first population one or more soybean plants or seeds comprising said molecular marker; and
crossing or selfing said selected one or more soybean plants or plants grown from said selected seeds to produce a second population of progeny soybean plants or seeds comprising said ASR resistance locus 10, 11, 12, or 13 in haplotype window 2.
2. The method of claim 1 , wherein said locus linked to ASR resistance haplotype window 2 is a locus obtainable from a soybean accession selected from the group consisting of PI164885, PI165524, PI166028, PI189968, PI200488, PI227331, PI229333, PI341252, PI407858, PI423852, PI437437A, PI437740B, PI437982, PI507142, PI561289, PI561330A, PI561337, PI561377, PI567093B, PI567104B, PI567108B, PI567129, PI567174C, PI567175C, PI567560, PI567675, PI567692, PI567718, PI587914B, PI587922A, PI587935A, PI588000, PI588034, PI594525, PI594538A, PI603296, PI603612, PI603704A, and PI628870.
3. The method of claim 1 , wherein said genotyping comprises detecting SNP marker NS0124935 with probes having SEQ ID NOs: 164, 165, or complements thereof.
4. The method of claim 1 , wherein said second population of soybean plants or seeds further comprise one or more loci associated with ASR resistance selected from the group consisting of ASR resistance loci 1, 2, 3, 4, and ASR resistance haplotype window 1.
5. The method of claim 4 , wherein said second population of soybean plants or seeds further comprise ASR resistance locus 1 mapped within 10 centimorgans or less from a SNP marker selected from the group consisting of
NS0093250 capable of being identified with probes with SEQ ID NOs: 100 and 101,
NS0119710 capable of being identified with probes with SEQ ID NOs: 102 and 103,
NS0103004 capable of being identified with probes with SEQ ID NOs: 104 and 105,
NS0099454 capable of being identified with probes with SEQ ID NOs: 106 and 107,
NS0102630 capable of being identified with probes with SEQ ID NOs: 108 and 109,
NS0102915 capable of being identified with probes with SEQ ID NOs: 110 and 111,
NS0102913 capable of being identified with probes with SEQ ID NOs: 112 and 113,
NS0123728 capable of being identified with probes with SEQ ID NOs: 114 and 115,
NS0129943 capable of being identified with probes with SEQ ID NOs: 116 and 117,
NS0102168 capable of being identified with probes with SEQ ID NOs: 118 and 119,
NS0092723 capable of being identified with probes with SEQ ID NOs: 120 and 121,
NS0098177 capable of being identified with probes with SEQ ID NOs: 122 and 123,
NS0127343 capable of being identified with probes with SEQ ID NOs: 124 and 125, and
NS0101121 capable of being identified with probes with SEQ ID NOs: 126 and 127.
6. The method of claim 4 , wherein said second population of soybean plants or seeds further comprise ASR resistance locus 3 mapped within 10 centimorgans or less from one or more SNP markers selected from the group consisting of
NS0099746 capable of being identified with probes with SEQ ID NOs: 128 and 129,
NS0123747 capable of being identified with probes with SEQ ID NOs: 130 and 131,
NS0126598 capable of being identified with probes with SEQ ID NOs: 132 and 133,
NS0128378 capable of being identified with probes with SEQ ID NOs: 134 and 135,
NS0096829 capable of being identified with probes with SEQ ID NOs: 136 and 137,
NS0125408 capable of being identified with probes with SEQ ID NOs: 138 and 139,
NS0098902 capable of being identified with probes with SEQ ID NOs: 140 and 141,
NS0099529 capable of being identified with probes with SEQ ID NOs: 142 and 143,
NS0097798 capable of being identified with probes with SEQ ID NOs: 144 and 145,
NS0137477 capable of being identified with probes with SEQ ID NOs: 146 and 147,
NS0095322 capable of being identified with probes with SEQ ID NOs: 148 and 149,
NS0136101 capable of being identified with probes with SEQ ID NOs: 150 and 151,
NS0098982 capable of being identified with probes with SEQ ID NOs: 152 and 153,
NS0103749 capable of being identified with probes with SEQ ID NOs: 154 and 155,
NS0118897 capable of being identified with probes with SEQ ID NOs: 156 and 157,
NS0119715 capable of being identified with probes with SEQ ID NOs: 158 and 159, and
NS0130920 capable of being identified with probes with SEQ ID NOs: 160 and 161.
7. The method of claim 4 , wherein said second population of soybean plants or seeds further comprise an ASR resistance locus linked to haplotype window 1 on chromosome 13 comprising a locus within 10 centimorgans or less from SNP marker NS0103033, is capable of being identified with probes with SEQ ID NOs: 162, 163, and complements thereof.
8. The method of claim 1 , wherein said second population of soybean plants or seeds comprise a slow rust phenotype.
9. The method of claim 1 , wherein said ASR resistance is resistance to Phakopsora pachyrhizi or Phakopsora meibomiae.
10. The method of claim 1 , wherein said second population of soybean plants or seeds comprise one or more agronomic traits selected from the group consisting of herbicide tolerance, increased yield, insect control, fungal disease resistance, virus resistance, nematode resistance, bacterial disease resistance, mycoplasma disease resistance, modified oils production, high oil production, high protein production, germination and seedling growth control, enhanced animal and human nutrition, lower raffinose, environmental stress resistance, increased digestibility, production of industrial enzymes, production of pharmaceutical proteins, production of pharmaceutical peptides, production of pharmaceutical small molecules, improved processing traits, improved flavor, improved nitrogen fixation, improved hybrid seed production, reduced allergenicity, and improved production of biopolymers and biofuels.
11. The method of claim 1 , wherein said second population of soybean plants or seeds comprise one or more traits selected from the group consisting of insect resistance, glyphosate resistance, resistance to Soybean Cyst Nematode, resistance to Meloidogyne javanica , resistance to Meloidogyne arenaria , resistance to Meloidogyne hapla , resistance to Meloidogyne incognita , resistance to Diaporthe phaseolorum var. caulivora , and resistance to Corynespora cassiicola.
12. The method of claim 1 , wherein said second population of soybean plants or seeds are transgenic.
13. The method of claim 1 , wherein said genotyping involves detecting one or more markers selected from the group consisting of a simple sequence repeat (SSR) marker, an amplified fragment length polymorphism (AFLP) marker, a restriction fragment length polymorphism (RFLP) marker, a random amplified polymorphic DNA (RAPD) marker, a SNP, and microarray transcription profile.
14. The method of claim 1 , wherein said genotyping comprises detecting a molecular marker located within 7.5 centimorgans of said SNP marker NS0124935.
15. The method of claim 1 , wherein said genotyping comprises detecting a molecular marker located within 5 centimorgans of said SNP marker NS0124935.
16. The method of claim 1 , wherein said genotyping comprises detecting a molecular marker located within 2.5 centimorgans of said SNP marker NS0124935.
17. The method of claim 1 , wherein said genotyping comprises detecting SNP marker NS0124935.
18. The method of claim 1 , wherein said genotyping involves one or more methods selected from the group consisting of polymerase chain reaction (PCR), Ligase Chain Reaction (LCR), Oligonucleotide Ligation Assay (OLA), allele-specific oligomers, branched DNA technology, transcription-based amplification systems, isothermal amplification methods, and Single Strand Conformation Polymorphism (SSCP) analysis.
19. The method of claim 1 , wherein said genotyping comprises a primer-extension assay.
20. The method of claim 1 , wherein said genotyping uses one or more methods selected from the group consisting of oligonucleotide hybridization, Taq-Man assay, molecular beacon, electronic dot blot assay, GOOD assay and denaturing high-performance liquid chromatography.
21. The method of claim 1 , where said selecting comprises selecting a genotype selected from the group consisting of ASR resistance locus 10, locus 11, locus 12, and locus 13.