Blood type checking reagent containing lectin
Lectin for checking a blood type is extracted from seeds of bitter gourd, balsam pear, or Momordica charantia . A blood type checking reagent includes the lectin. The lectin extracted from seeds of bitter gourd preferably has a molecular weight between 100,000 and 170,000 measured with poly-acrylamide gel electrophoresis in existence of sodium dodecyl sulfate (SDS-PAGE). A method of checking a blood type includes the steps of: extracting lectin from seeds of bitter gourd, balsam pear, or Momordica charantia ; preparing a blood type checking reagent containing the lectin; and checking a blood type using the blood type checking reagent.
1 . A blood type checking reagent, comprising lectin extracted from seeds of bitter gourd (Momordica charantia) through the steps of:
grinding the seeds of bitter gourd to obtain first power;
processing the first powder to obtain second powder;
adding the second powder to normal saline solution containing gelatin to obtain a mixture; and
centrifuging the mixture to obtain supernatant as the lectin.
2 . The blood type checking reagent according to claim 1 , further comprising the steps of:
adding the first powder into distilled water to obtain a first mixture;
filtering the first mixture to obtain filtrate;
centrifuging the filtrate to obtain first supernatant;
adding the first supernatant to ethanol to obtain a second mixture;
removing second supernatant of the second mixture to obtain first deposit;
centrifuging the first deposit to obtain second deposit; and
drying the second deposit to obtain the second powder.
3 . The blood type checking reagent according to claim 1 , wherein said lectin has a molecular weight between 100,000 and 170,000 measured with poly-acrylamide gel electrophoresis in existence of sodium dodecyl sulfate (SDS-PAGE).