IP Library › Granted Patent US 12,428,635
Granted Patent B2
US 12,428,635 · App. 18/653,435 · Granted Sep 30, 2025

Isotachophoresis for purification of nucleic acids

Inventors: Lewis A. Marshall (Pleasanton, CA); Amy L. Hiddessen (Pleasanton, CA); Nathan P. Hoverter (Pleasanton, CA); Klint A Rose (Pleasanton, CA); Juan G. Santiago (Pleasanton, CA)
Assignee: Purigen Biosystems, Inc.
C12N15/101B01L3/502753C12N15/10C12Q1/6806G01N27/4473G01N27/44739G01N27/44791G01N27/44795B01L3/502738B01L2300/0627B01L2300/0867B81B1/004B81B7/0087B81B2201/0278B81B2201/05B81B2203/0338G01N27/44704
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Quick Facts
Patent No.
US 12,428,635
App. No.
18/653,435
Granted
Sep 30, 2025
Kind
B2
Abstract

The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.

Claims (22)

1. A microfluidic chip comprising two or more fluidic channels each in fluid communication with a sample reservoir configured to receive a sample, a first buffer reservoir configured to receive a buffer, a second buffer reservoir configured to receive a second buffer, a third buffer reservoir configured to receive a third buffer and an elution reservoir configured to receive an eluate, wherein the two or more fluidic channels each comprise a branched fluidic channel comprising a loading section and an isotachophoresis (“ITP”) section wherein the ITP section comprises a leading electrolyte branch and an elution branch, meeting at a branch point.

2. The microfluidic chip according to claim 1 , wherein the loading section comprises:

(1) a trailing electrolyte well fluidically connected to a sample input region of the fluidic channel; and

(2) a sample input well fluidically connected to the sample input region of the fluidic channel.

3. The microfluidic chip according to claim 2 , wherein the each fluidic channel further comprises a capillary barrier positioned between the sample input region of the fluidic channel and the ITP section.

4. The microfluidic chip according to claim 2 , wherein the loading section further comprises a trailing capillary barrier positioned between the trailing electrolyte well and the sample input well, and a gas channel connected to the trailing capillary barrier and to a pneumatic pressure port.

5. The microfluidic chip according to claim 1 , wherein the leading electrolyte branch comprises a leading electrolyte well fluidically connected to the fluidic channel.

6. The microfluidic chip according to claim 1 , wherein the elution branch comprises a sample elution well fluidically connected to the fluidic channel.

7. The microfluidic chip according to claim 1 , wherein the ITP section further comprises a leading electrolyte buffering well fluidically connected to the leading electrolyte well through a leading electrolyte buffering channel.

8. The microfluidic chip according to claim 7 , wherein the leading electrolyte buffering channel comprises a leading capillary barrier and a gas channel connected to the leading capillary barrier and to a pneumatic pressure port.

9. The microfluidic chip according to claim 8 , wherein the elution branch further comprises an elution buffering well fluidically connected to the elution well through an elution buffering channel.

10. The microfluidic chip according to claim 9 , wherein the elution buffering channel comprises an elution capillary barrier, and a gas channel connected to the elution capillary barrier and to a pneumatic pressure port.

11. The microfluidic chip according to claim 9 , wherein the elution buffering channel comprises an elution capillary barrier, and a gas channel connected to the elution capillary barrier and to a pneumatic pressure port.

12. The microfluidic chip according to claim 1 , wherein the microfluidic chip comprises 8 fluidic channels.

13. A method comprising:

introducing a sample into a sample reservoir of a microfluidic chip comprising two or more fluidic channels each in fluid communication with the sample reservoir, a first buffer reservoir configured to receive a buffer, a second buffer reservoir configured to receive a second buffer, a third buffer reservoir configured to receive a third buffer and an elution reservoir configured to receive an eluate, wherein the two or more fluidic channels each comprise a branched fluidic channel comprising a loading section and an isotachophoresis (“ITP”) section wherein the ITP section comprises a leading electrolyte branch and an elution branch, meeting at a branch point.

14. The method according to claim 13 , wherein the method further comprises introducing a first buffer into the first buffer reservoir.

15. The method according to claim 14 , wherein the method further comprises introducing a second buffer into the second buffer reservoir.

16. The method according to claim 15 , further comprising performing isotachophoresis on the sample.

17. The method according to claim 13 , wherein the sample comprises nucleic acids.

18. The method according to claim 13 , wherein the method further comprises preparing the sample.

19. The method according to claim 13 , wherein the sample comprises a lysed sample.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 16, 2024
From: MARSHALL, LEWIS A.; HIDDESSEN, AMY L.; HOVERTER, NATHAN P.; ROSE, KLINT A.; SANTIAGO, JUAN G.
To: PURIGEN BIOSYSTEMS, INC.
Reel/Frame 068395/0729 →
SECURITY INTEREST Recorded May 24, 2024
From: BIONANO GENOMICS, INC.; BIODISCOVERY, LLC; LINEAGEN, INC.; PURIGEN BIOSYSTEMS, INC.
To: JGB COLLATERAL, LLC
Reel/Frame 067529/0286 →
Continuity (6)
Continuation 18141641 · May 1, 2023
Continuation 16997614 · Aug 19, 2020
Continuation 16046560 · Jul 26, 2018
Continuation PCTUS2017015519 · Jan 28, 2017
Provisional Application 62288930 · Jan 29, 2016
Related Publication 20240301395A1 · Sep 12, 2024
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