SCARLESS TEMPLATE-FREE ENZYMATIC SYNTHESIS OF POLYNUCLEOTIDES

Abstract

Methods are provided for template-free enzymatic synthesis of polynucleotides on a solid support using double cleavage to produce scarless polynucleotides. In particular, methods are disclosed that use an initiator that comprises a cleavable group, which can be cleaved by a small molecule chemical agent, adjusting pH, heat, or photocleaved by illumination with light to release polynucleotides from a solid support after enzymatic synthesis is completed, and an enzymatic cleavage site, which can be cleaved by a cleavage enzyme to remove polynucleotide scars.

Inventors

Applicants

IPC Classification

B01J 19/ 00 A I C12P 19/ 34 A I C40B 50/ 14 A I