Acid soluble proteins from micellar casein
View Patent ↗The present invention relates to a milk fraction obtainable by acidification of micellar casein and separation from precipitated casein named acid soluble protein from micellar casein. It was found that the milk fraction and especially certain sub-fractions thereof are bioactive and promote GLP-1 release in vitro. Based on these results, acid soluble protein from micellar casein may be useful in the treatment and the prevention of diabetes type II, obesity and may further be added to formulas directed at other purposes addressing the gastro-intestinal tract.
1. A method of obtaining fractions of acid soluble proteins of micellar casein, comprising the steps of
separating micellar casein and whey proteins,
acidifying micellar casein to a pH below 6,
separating acid soluble proteins from casein by a step selected from the group consisting of centrifugation and ultrafiltration, and
separating specific sub-fractions of acid soluble proteins according to the hydrophobic properties of the sub-fractions.
2. The method of claim 1 wherein specific sub-fractions of acid soluble proteins are separated by hydrophobic interaction chromatography or hydrophobic interaction liquid chromatography.
3. The method of claim 1 wherein specific sub-fractions of acid soluble proteins are separated according to the hydrophobic properties of the sub-fractions by varying the ratio of a first buffer comprising trifluoroacetic acid in water to a second buffer comprising acetonitrile, wherein specific ratios of the first buffer to the second buffer define specific sub-fractions of acid soluble proteins.