Lentiviral LTR-deleted vector
A vector capable of transducing non-dividing and/or slowly dividing cells is provided, wherein the vector is a lentiviral LTR-deleted vector. Also provided is a method for producing a protein of interest in a non-dividing or slowly dividing cell by transducing the cell with a lentiviral LTR-deleted vector and expressing the protein of interest in the cell. In addition, target cells containing the lentiviral LTR-deleted vector are provided.
1. A lentiviral vector capable of transducing a non-dividing or slowly-dividing cell, said vector comprising a lentiviral LTR-deleted vector.
2. The lentiviral vector according to claim 1 , further comprising a nucleotide sequence encoding a protein of interest.
3. A target cell in vitro comprising the lentiviral vector of claim 1 .
4. A method for producing a protein of interest in a non-dividing or slowly-dividing cell, comprising the steps of:
a) transducing the cell with the lentiviral vector according to claim 2 ; and
b) expressing the protein of interest in the cell.
5. A target cell in vitro comprising the lentiviral vector of claim 2 .
6. A method of performing gene delivery on a target cell comprising the steps of:
a) transducing the target cell with the lentiviral vector according to claim 2 ; and
b) delivering the nucleotide sequence to the target cell.
7. The method according to claim 4 , wherein the non-dividing cell is a neuron.
8. A lentiviral LTR-deleted vector.
9. The lentiviral LTR-deleted vector according to claim 8 , further comprising a nucleotide sequence of interest.