Extracts obtained from cell line cultures from plants belonging to the Oleaceae family (e.g.
View Patent ↗The present invention refers to the use of extracts from selected and stabilized cell lines comprising phenylpropanoids with high anti-oxidant capacity having a verbascoside titre of between 20% and 90% and a chromophore-free fraction of between 80% and 10%, in human and veterinary medicine, and for nutritional and cosmetic purposes.
1. A Syringa vulgaris plant cell line extract comprising between 30% and 90% by weight phenylpropanoids and between 70% and 10% by weight of a chromophore-free fraction consisting essentially of oligo- and polysaccharide, protein and lipid molecules obtained by:
a) subjecting a biomass obtained following culture of said plant cell line to a thermal enzyme deactivating treatment at a temperature between 50° C. and 150° C.;
b) homogenizing the product obtained by step a) to provide an aqueous phase containing cell residue;
c) separating the aqueous phase from the cell residue by means of gravity filtration, pressure filtration or centrifugation;
d) performing an extraction of the aqueous phase obtained following step c) with hydrophobic interaction resin; and
e) recovering the extract from the resin by means of elution with a mixture of alcohols having up to 5 carbon atoms, in water in percentages varying from 30% up to just under 100% by volume,
wherein the plant cell line is a stabilized cell line of Syringa vulgaris cell line IRB-SV25/B (DSM:16857).
2. The extract according to claim 1 , comprising between 30% and 60% by weight phenylpropanoids and between 70% and 40% by weight of said chromophore-free fraction.
3. The extract according to claim 1 , wherein the phenyl-propanoids comprise between 50% and 20% by weight isoverbascoside with respect to the total mass of phenylpropanoids.
4. The extract according to claim 1 , wherein the phenyl-propanoids comprise between 45% and 65% by weight isoverbascoside with respect to the total mass of phenylpropanoids.
5. The extract according to claim 1 , wherein the phenyl-propanoids are represented by the following general formula:
wherein:
R1 is a hydrogen atom or a monosaccharide with 5 or 6 carbon atoms and preferably rhamnose;
R2 is a hydrogen atom or is a caffeoyl (A) or feruloyl (B) group;
R3 is a hydrogen atom or a monosaccharide with 5 or 6 carbon atoms or a disaccharide with 10 to 12 carbon atoms or is a caffeoyl (A) or feruloyl (B) group;
R4 is a hydrogen atom or an alkyl group, preferably methyl, or a hydroxyl group;
R5 is a hydrogen atom or an alkyl group, preferably methyl, or a hydroxyl group, either free or condensed with the alcohol group at position C2 of the monosaccharide group bound to it, in order to form a 1,4 dioxane ring;
R6 is a hydrogen atom or an alkyl group, preferably methyl;
when R3 is a caffeoyl (A) or feruloyl (B) group,
then R2 is always a hydrogen atom and vice versa.
6. A formulation comprising the extract according to claim 1 as an active ingredient further comprising pharmaceutically, nutritionally or cosmetically acceptable excipients for human and veterinary use.
7. The formulation according to claim 6 for systemic, parenteral, oral, rectal, inhalatory, topical, transdermal, intravenous, intramuscular or subcutaneous administration.
8. The formulation according to claim 7 , wherein the form for oral administration is selected from the group consisting of tablets, lozenges, capsules, powders, solutions, suspensions and a nebulised form.
9. The formulation according to claim 7 wherein the form for administration by inhalation is selected from the group consisting of powders, solutions and suspensions.
10. The formulation according to claim 7 , wherein the form for topical administration is an emulsion, gel or compatible solutions for dermal and mucosal use and eye washes.
11. A Syringa vulgaris plant cell line extract according to claim 1 as a cosmetic product.
12. A Syringa vulgaris plant cell line extract according to claim 1 as a nutritional product.
13. A Syringa vulgaris plant cell line IRB-SV25/B.
14. A Syringa vulgaris plant cell line IRB-SV25/B (DSM:16857) extract comprising between 30% and 90% by weight phenylpropanoids and between 70% and 10% by weight of a chromophore-free fraction consisting essentially of oligo- and polysaccharide, protein and lipid molecules, said extract having an antioxidant activity, with respect to superoxide radical, expressed as IC50 of less than 0.3 micro gram/ml.