IP Library Granted Patent US 7,718,199
Granted Patent B2
US 7,718,199 · App. 11/455,501 · Granted May 18, 2010

Extracts obtained from cell line cultures from plants belonging to the Oleaceae family (e.g.

Assignee: I.R.B. Istituto di Ricerche Biotecnologiche S.r.l.
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Quick Facts
Patent No.
US 7,718,199
App. No.
11/455,501
Granted
May 18, 2010
Kind
B2
Abstract

The present invention refers to the use of extracts from selected and stabilized cell lines comprising phenylpropanoids with high anti-oxidant capacity having a verbascoside titre of between 20% and 90% and a chromophore-free fraction of between 80% and 10%, in human and veterinary medicine, and for nutritional and cosmetic purposes.

Claims (29)

1. A Syringa vulgaris plant cell line extract comprising between 30% and 90% by weight phenylpropanoids and between 70% and 10% by weight of a chromophore-free fraction consisting essentially of oligo- and polysaccharide, protein and lipid molecules obtained by:

a) subjecting a biomass obtained following culture of said plant cell line to a thermal enzyme deactivating treatment at a temperature between 50° C. and 150° C.;

b) homogenizing the product obtained by step a) to provide an aqueous phase containing cell residue;

c) separating the aqueous phase from the cell residue by means of gravity filtration, pressure filtration or centrifugation;

d) performing an extraction of the aqueous phase obtained following step c) with hydrophobic interaction resin; and

e) recovering the extract from the resin by means of elution with a mixture of alcohols having up to 5 carbon atoms, in water in percentages varying from 30% up to just under 100% by volume,

wherein the plant cell line is a stabilized cell line of Syringa vulgaris cell line IRB-SV25/B (DSM:16857).

2. The extract according to claim 1 , comprising between 30% and 60% by weight phenylpropanoids and between 70% and 40% by weight of said chromophore-free fraction.

3. The extract according to claim 1 , wherein the phenyl-propanoids comprise between 50% and 20% by weight isoverbascoside with respect to the total mass of phenylpropanoids.

4. The extract according to claim 1 , wherein the phenyl-propanoids comprise between 45% and 65% by weight isoverbascoside with respect to the total mass of phenylpropanoids.

5. The extract according to claim 1 , wherein the phenyl-propanoids are represented by the following general formula:

wherein:

R1 is a hydrogen atom or a monosaccharide with 5 or 6 carbon atoms and preferably rhamnose;

R2 is a hydrogen atom or is a caffeoyl (A) or feruloyl (B) group;

R3 is a hydrogen atom or a monosaccharide with 5 or 6 carbon atoms or a disaccharide with 10 to 12 carbon atoms or is a caffeoyl (A) or feruloyl (B) group;

R4 is a hydrogen atom or an alkyl group, preferably methyl, or a hydroxyl group;

R5 is a hydrogen atom or an alkyl group, preferably methyl, or a hydroxyl group, either free or condensed with the alcohol group at position C2 of the monosaccharide group bound to it, in order to form a 1,4 dioxane ring;

R6 is a hydrogen atom or an alkyl group, preferably methyl;

when R3 is a caffeoyl (A) or feruloyl (B) group,

then R2 is always a hydrogen atom and vice versa.

6. A formulation comprising the extract according to claim 1 as an active ingredient further comprising pharmaceutically, nutritionally or cosmetically acceptable excipients for human and veterinary use.

7. The formulation according to claim 6 for systemic, parenteral, oral, rectal, inhalatory, topical, transdermal, intravenous, intramuscular or subcutaneous administration.

8. The formulation according to claim 7 , wherein the form for oral administration is selected from the group consisting of tablets, lozenges, capsules, powders, solutions, suspensions and a nebulised form.

9. The formulation according to claim 7 wherein the form for administration by inhalation is selected from the group consisting of powders, solutions and suspensions.

10. The formulation according to claim 7 , wherein the form for topical administration is an emulsion, gel or compatible solutions for dermal and mucosal use and eye washes.

11. A Syringa vulgaris plant cell line extract according to claim 1 as a cosmetic product.

12. A Syringa vulgaris plant cell line extract according to claim 1 as a nutritional product.

13. A Syringa vulgaris plant cell line IRB-SV25/B.

14. A Syringa vulgaris plant cell line IRB-SV25/B (DSM:16857) extract comprising between 30% and 90% by weight phenylpropanoids and between 70% and 10% by weight of a chromophore-free fraction consisting essentially of oligo- and polysaccharide, protein and lipid molecules, said extract having an antioxidant activity, with respect to superoxide radical, expressed as IC50 of less than 0.3 micro gram/ml.

Assignments (3)
MERGER Recorded May 30, 2019
From: I.R.B. ISTITUTO DI RICERCHE BIOTECNOLOGICHE S.P.A.
To: CRODA ITALIANA S.P.A.
Reel/Frame 049317/0958 →
CHANGE OF NAME Recorded May 28, 2019
From: I.R.B. ISTITUTO DI RICERCHE BIOTECNOLOGICHE S.R.L.
To: I.R.B. ISTITUTO DI RICERCHE BIOTECNOLOGICHE S.P.A.
Reel/Frame 049289/0305 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 19, 2006
From: DAL MONTE, RENZO; DAL TOSO, ROBERTO; MINGHETTI, ANACLETO; CRESPI PERELLINO, NICOLETTA; PRESSI, GIOVANNA
To: I.R.B. ISTITUTO DI RICERCHE BIOTECNOLOGICHE S.R.L.
Reel/Frame 018012/0124 →
Priority Claims (2)
EP 05425442.0 · Jun 20, 2005 · regional
EP 05425586.4 · Aug 8, 2005 · regional
Continuity (1)
Related Publication 20070004011A1 · Jan 4, 2007