Capsular polysaccharide solubilisation and combination vaccines
Precipitated bacterial capsular polysaccharides can be efficiently re-solubilized using alcohols as solvents. The invention provides a process for purifying a bacterial capsular polysaccharide, comprising the steps of (a) precipitation of said polysaccharide, followed by (b) solubilization of the precipitated polysaccharide using ethanol. CTAB can be used for step (a). The material obtained, preferably following hydrolysis and sizing, can be conjugated to a carrier protein and formulated as a vaccine. Also, in vaccines comprising saccharides from both serogroups A and C, the invention provides that the ratio (w/w) of MenA saccharide:MenC saccharide is >1.
1. A method for conjugating a bacterial capsular polysaccharide to a carrier protein, comprising
(a) purifying the polysaccharide, comprising the steps of (i) precipitation of the polysaccharide using one or more cationic detergents, followed by (ii) solubilisation of the precipitated polysaccharide using an alcohol, and
(b) conjugating the polysaccharide to a carrier protein,
wherein the bacterial capsular polysaccharide is from Neisseria meningitidis serogroup A, Neisseria meningitidis serogroup W135, Neisseria meningitidis serogroup Y, Haemophilus influenzae or Streptococcus pneumoniae , wherein the method further comprises a step of hydrolysis to form oligosaccharides prior to step (b).
2. The method of claim 1 , wherein the cationic detergent(s) have the following general formula:
wherein:
R 1 , R 2 and R 3 are the same or different and each signifies alkyl or aryl; or R 1 and R 2 together with the nitrogen atom to which these are attached form a 5- or 6-membered saturated heterocyclic ring, and R 3 signifies alkyl or aryl; or R 1 , R 2 and R 3 together with the nitrogen atom to which these are attached form a 5- or 6-member heterocyclic ring, unsaturated at the nitrogen atom,
R 4 signifies alkyl or aryl, and
X − signifies an anion.
3. The method of claim 1 or claim 2 , wherein the one more cationic detergents comprise a cetyltrimethylammonium salt, a tetrabutylammonium salt, a myristyltrimethylammonium salt or hexadimethrine bromide.
4. The method of claim 3 , wherein the cationic detergent is cetyltrimethylammonium bromide.
5. The method of claim 1 or claim 2 , wherein the alcohol used in step (b) comprises ethanol.
6. The method of claim 3 , wherein the alcohol used in step (b) comprises ethanol.
7. The method of claim 5 , wherein the ethanol has a final concentration of between 50% and 95%.
8. The method of claim 6 , wherein the ethanol has a final concentration of between 50% and 95%.
9. The method of claim 1 or claim 2 , further comprising the step of (c) treating the polysaccharide obtained in step (b) to remove contaminants.
10. The method of claim 6 , further comprising the step of (c) treating the polysaccharide obtained in step (b) to remove contaminants.
11. The method of claim 9 , wherein step (c) comprises a step of filtration.
12. The method of claim 10 , wherein step (c) comprises a step of filtration.
13. The method of claim 11 , wherein the filtration is one or more of depth filtration, filtration through activated carbon, size filtration and ultrafiltration.
14. The method of claim 12 , wherein the filtration is one or more of depth filtration, filtration through activated carbon, size filtration and ultrafiltration.
15. The method of claim 1 or claim 2 , wherein the polysaccharide obtained in step (b) or step (c) is then precipitated.
16. The method of claim 10 , wherein the polysaccharide obtained in step (b) or step (c) is then precipitated.
17. The method of claim 1 , further comprising the step of sizing in order to remove short-length oligosaccharides.
18. The method of claim 10 , further comprising the step of sizing in order to remove short-length oligosaccharides.
19. The method of claim 15 , further comprising the step of sizing in order to remove short-length oligosaccharides.
20. The method of claim 16 , further comprising the step of sizing in order to remove short-length oligosaccharides.
21. The method of claim 1 , further comprising the step of mixing with other biological molecules.
22. The method of claim 21 , wherein the further biological molecules are selected from the group consisting of: saccharide antigens from serogroup C of Neisseria meningitidis , and protein antigens from serogroup B of Neisseria meningitidis .
23. The method of claim 21 , wherein the other biological molecules are one or more Neisseria meningitidis saccharide antigens from serogroup A, C, W135 or Y, or from a mixture thereof.
24. The method of claim 1 , further comprising the step(s) of vaccine formulation.
25. The method of claim 24 , wherein the step(s) of vaccine formulation comprises mixing the saccharide antigen(s) with an adjuvant.
26. The method of claim 24 , wherein the adjuvant is an aluminum phosphate adjuvant, an aluminum hydroxide adjuvant or a combination thereof.
27. A method for conjugating to a carrier protein bacterial capsular polysaccharide that has been precipitated using one or more cationic detergents and then solubilized using ethanol as a solvent,
wherein the capsular polysaccharide is from Neisseria meningitidis serogroup A, Neisseria meningitidis serogroup W135, Neisseria meningitidis serogroup Y, Haemophilus influenzae or Streptococcus pneumoniae , wherein the ethanol is in the form of at least 95:5 ethanol:water.
28. The method of claim 27 , wherein the carrier protein is selected from the group consisting of: a diphtheria toxoid, a tetanus toxoid and a CRM 197 diphtheria toxoid.
29. The method of claim 27 , wherein the ethanol is in the form of a 95:5 ethanol:water mixture.