Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof
Provided are fabrication, characterization and application of a nanodisk electrode, a nanopore electrode and a nanopore membrane. These three nanostructures share common fabrication steps. In one embodiment, the fabrication of a disk electrode involves sealing a sharpened internal signal transduction element (“ISTE”) into a substrate, followed by polishing of the substrate until a nanometer-sized disk of the ISTE is exposed. The fabrication of a nanopore electrode is accomplished by etching the nanodisk electrode to create a pore in the substrate, with the remaining ISTE comprising the pore base. Complete removal of the ISTE yields a nanopore membrane, in which a conical shaped pore is embedded in a thin membrane of the substrate.
1. A nanopore membrane, comprising:
a membrane having a thickness, with a first, exterior, side and a second, interior, side, with the first side being opposite the second side, wherein the membrane is made from a material selected from the group consisting of glass, Si, SiO 2 , Si 3 N 4 , quartz, alumina, nitrides, metals and polymers; and
a nanopore extending through the membrane, thus forming a channel connecting from the first side to the second side of the membrane, wherein the nanopore has a first opening that opens to the first side of the membrane, a second opening that opens to the second side of the membrane and a depth, wherein the surface properties of at least one of: the first side of the membrane; the second side of the membrane; and the channel connecting the first side to the second side of the membrane are modified to change at least one of an electrical charge density, hydrophobicity and hydrophilicity property thereof, with the first side of the membrane being modified with a first entity, and the channel connecting the first side to the second side of the membrane being modified with a second entity which is different from the first entity.
2. The nanopore membrane of claim 1 , wherein the nanopore is of a truncated conical shape and wherein a radius of the first opening of the nanopore is smaller than a radius of the second opening of the nanopore.
3. The nanopore membrane of claim 1 , wherein the radius of the first opening of the nanopore ranges from about 2 nm to 500 nm.
4. The nanopore membrane of claim 1 , wherein the radius of the first opening of the nanopore is larger than 500 nm.
5. The nanopore membrane of claim 1 , wherein the first entity is a reactive silane with an inert terminus, and the second entity is a saline that comprises a first functional group able to react with the membrane and a second functional group able to react with a sensor element able to selectively bind a target analyte.
6. The nanopore membrane of claim 1 , wherein the first entity is Cl(Me) 2 Si(CH 2 ) 3 CN, and the second entity is EtO(Me) 2 Si(CH 2 ) 3 NH 2 .
7. A nanopore membrane comprising:
a membrane having a thickness, with a first, exterior, side and a second, interior, side, with the first side being opposite the second side, wherein the membrane is made from a material selected from the group consisting of glass, Si, SiO 2 , Si 3 N 4 , quartz, alumina, nitrides, metals and polymers; and
a nanopore extending through the membrane, thus forming a channel connecting the first side to the second side of the membrane, wherein the nanopore has a first opening that opens to the first side of the membrane, a second opening that opens to the second side of the membrane and a depth;
wherein the membrane is a bottom wall of a capillary which is sealed to produce the bottom wall.
8. The nanopore membrane of claim 7 , wherein the surface properties of at least one of: the first side of the membrane; the second side of the membrane; and the channel connecting the first side to the second side of the membrane are modified to change at least one of an electrical charge density, hydrophobicity and hydrophilicity property thereof.
9. The nanopore membrane of claim 7 , wherein the capillary is made from glass or quartz.
10. The nanopore membrane of claim 7 , wherein the nanopore is of a truncated conical shape and wherein a radius of the first opening of the nanopore is smaller than a radius of the second opening of the nanopore.
11. The nanopore membrane of claim 7 , wherein the radius of the first opening of the nanopore ranges from about 2 nm to 500 nm.
12. The nanopore membrane of claim 7 , wherein the radius of the first opening of the nanopore is larger than 500 nm.
13. The nanopore membrane of claim 7 , wherein the first side of the membrane is modified with a first entity, and the channel connecting the first side to the second side of the membrane is modified with a second entity.
14. The nanopore membrane of claim 13 , wherein the first entity is a reactive silane with an inert terminus, and the second entity is a saline that comprises a first functional group able to react with the membrane and a second functional group able to react with a sensor element able to selectively bind a target analyte.
15. The nanopore membrane of claim 14 , wherein the first entity is Cl(Me) 2 Si(CH 2 ) 3 CN, and the second entity is EtO(Me) 2 Si(CH 2 ) 3 NH 2 .
16. The nanopore membrane of claim 13 , wherein the first entity and the second entity are the same.