Polypeptides having xylanase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. An isolated polynucleotide comprising a nucleotide sequence that encodes a polypeptide having xylanase activity, selected from the group consisting of:
(a) a polynucleotide encoding a polypeptide comprising an amino acid sequence having at least 98% identity to amino acids 20 to 407 of SEQ ID NO: 2;
(b) a polynucleotide encoding a polypeptide comprising nucleotides 58 to 1439 of SEQ ID NO: 1; and
(c) a polynucleotide encoding a fragment of SEQ ID NO: 2 having xylanase activity.
2. The polynucleotide of claim 1 , which encodes a polypeptide comprising an amino acid sequence having at least 99% identity to amino acids 20 to 407 of SEQ ID NO: 2.
3. The polynucleotide of claim 1 , which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or amino acids 20 to 407 of SEQ ID NO: 2; or a fragment thereof having xylanase activity.
4. The polynucleotide of claim 3 , which encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO: 2 or amino acids 20 to 407 of SEQ ID NO: 2; or a fragment thereof having xylanase activity.
5. The polynucleotide of claim 1 , which is contained in plasmid pGEM-T-Ppin3 which is contained in E. coli DSM 22922.
6. A nucleic acid construct comprising the polynucleotide of claim 1 operably linked to one or more control sequences that direct the production of the polypeptide in an expression host.
7. A recombinant expression vector comprising the nucleic acid construct of claim 6 .
8. A recombinant host cell comprising the nucleic acid construct of claim 6 .
9. A method of producing a polypeptide having xylanase activity, comprising: (a) cultivating the recombinant host cell of claim 8 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
10. A method of producing a mutant of a parent Penicillium cell, comprising disrupting or deleting the polynucleotide encoding the polypeptide having xylanase activity of claim 1 , which results in the mutant producing less of the polypeptide than the parent cell.
11. A transgenic plant, plant part, or plant cell transformed with the polynucleotide of claim 1 encoding the polypeptide having xylanase activity.
12. A method of producing a polypeptide having xylanase activity, comprising: (a) cultivating the transgenic plant, plant part, or plant cell of claim 11 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
13. An isolated polynucleotide encoding a signal peptide comprising or consisting of amino acids 1 to 19 of SEQ ID NO: 2.
14. A nucleic acid construct comprising a gene encoding a protein operably linked to the polynucleotide of claim 13 , wherein the gene is foreign to the polynucleotide encoding the signal peptide.
15. A recombinant expression vector comprising the nucleic acid construct of claim 14 .
16. A recombinant host cell comprising the nucleic acid construct of claim 14 .
17. A method of producing a protein, comprising: (a) cultivating the recombinant host cell of claim 16 under conditions conducive for production of the protein; and (b) recovering the protein.