Compound arrays for sample profiling
View Patent ↗The invention provides arrays of compound for use in profiling samples. The arrays include compounds bind to components of the samples at relatively low affinities. The avidity of compounds binding to components of the samples can be increased by forming arrays such that multivalent components of the samples (e.g., antibodies or cells) can bind to more than one molecule of a compound at the same time. When a sample is applied to an array under such conditions, the compounds of the array bind to component(s) of the sample with significantly different avidities generating a profile characteristic of the sample.
1. An array to classify one or more states of health, comprising at least 100,000 different linear, unbranched peptides of between 10-30 natural amino acids in length, each of said at least 100,000 different linear, unbranched peptides having a unique amino acid sequence, each unique amino acid sequence chosen without regard to the identity of a particular target or natural ligand to the target, and each of said at least 100,000 different linear, unbranched peptides having a unique amino acid sequence occupying a distinct feature of the array, wherein each of said at least 100,000 different linear, unbranched peptides are immobilized onto a glass or a silicone surface of the array, and each of said at least 100,000 different linear, unbranched peptides peptides contain no cysteine residues with the optional exception of a terminal amino acid,
wherein each distinct feature of the array comprises at least two peptides of a same amino acid sequence,
wherein at least 50of said at least 100,000 different linear, unbranched peptides have dissociation constants of between 1 mM and 1 μM to at least one antibody in a sample and show a common pattern of binding for a health state of a patient to classify the one or more states of health of the patient, the one or more states of health of the patient including inflammation, cancer and infection, and
wherein the array does not include synbodies immobilized on the surface of the array.
2. The array of claim 1 , wherein said at least 100,000 different linear, unbranched peptides are 12 to 30 amino acids long.
3. The array of claim 1 , wherein said at least 100,000 different linear, unbranched peptides are 15 to 25 amino acids long.
4. The array of claim 1 , wherein said at least 100,000 different linear, unbranched peptides are 9 to 20 amino acids long.
5. The array of claim 1 , wherein said at least 100,000 different linear, unbranched peptides are 20 amino acids long.
6. The array of claim 1 , wherein said at least 100,000 different linear, unbranched peptides further comprise the synthetic amino acids ornithine or norleucine.
7. The array of claim 1 , wherein said at least 100,000 different linear, unbranched peptides consist of naturally occurring amino acids.
8. The array of claim 1 , wherein the array contains from 100,000 -1,000,000 peptides.
9. The array of claim 1 , wherein said at least two peptides of the same amino acid sequence on each distinct feature of the array have an average spacing of less than 4 nm apart.
10. The array of claim 1 , wherein said at least two peptides of the same amino acid sequence on each distinct feature of the array have an average spacing of 2-4 nm.
11. The array of claim 1 , wherein at least 100 of said at least 100,000 different linear, unbranched peptides on different features have dissociation constants of between 1 mM and 1 μM to at least one antibody in the patient sample.
12. The array of claim 1 , wherein at least 1000 of said at least 100,000different linear, unbranched peptides on different features have dissociation constants of between 1 mM and 1 μM to at least one antibody in the patient sample.
13. The array of claim 1 , wherein at least 1% of said at least 100,000 different linear, unbranched peptides on different features have dissociation constants of between 1 mM and 1 μM to at least one antibody in the patient sample.
14. The array of claim 1 , wherein the sample is a blood sample.
15. The array of claim 14 , wherein the sample is a serum sample.
16. The array of claim 1 , wherein the sample is a saliva sample.
17. The array of claim 1 , wherein the sample is a cerebrospinal fluid sample.