Nucleic acids encoding T2R bitter taste receptors
The invention provides nucleic acid and amino acid sequences for a novel family of taste transduction G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of taste transduction G-protein coupled receptors.
1. An isolated nucleic acid encoding a taste transduction G protein-coupled receptor having G protein-coupled receptor activity, the receptor comprising a polypeptide with greater than 90% amino acid sequence identity to SEQ ID NO:17, wherein said receptor detects bitter tastants, with the proviso that said isolated nucleic acid is not an isolated nucleic acid consisting of a genomic DNA.
2. The nucleic acid of claim 1 , wherein the polypeptide has greater than 95% amino acid sequence identity to SEQ ID NO:17.
3. The nucleic acid of claim 1 , wherein the polypeptide sequence is SEQ ID NO:17.
4. An isolated expression vector comprising the nucleic acid of claim 1 .
5. the isolated expression vector of claim 4 , wherein said polypepide comprises a sequence with at least 95% identity of SEQ ID NO:17.
6. The isolated expression vector of claim 4 , wherein the polypeptide sequence is SEQ ID NO:17.
7. An isolated cell comprising the expression vector of claim 4 .
8. The isolated cell of claim 7 , wherein said polypeptide comprises a sequence with at least 95% identity to SEQ ID NO:17.
9. The isolated cell of claim 7 , wherein said polypeptide sequence is SEQ ID NO:17.
10. The isolated nucleic acid of claim 1 , which comprises a cDNA.