Compositions and methods to detect various infectious organisms
The invention relates to compositions and methods for the detection of various infectious organisms, including heartworm ( Dirofilaria immitis ), Ehrlichia Canis, Anaplasma phagocytophilum , and Borrelia burgdorferi . More particularly, this invention relates to antibodies that bind to a heartworm antigen, the E. Canis gp36 polypeptide, the A. phagocytophilum p44 polypeptide, the B. burgdorferi OspA, OspC, OspF, p39, p41 and VlsE polypeptides, and uses thereof.
1. A method for detecting multiple disease antigens and/or antibodies in a sample, which method comprises
a) contacting said sample with a composition which composition comprises an Anaplasma phagocytophilum p44 polypeptide comprising the amino acid sequence set forth in SEQ ID NO:6 and at least one of the following reagents:
(1) an antibody against a heartworm ( Dirofilaria immitis ) antigen,
(2) an Ehrlichia canis gp36 polypeptide, and
(3) an antigenic composition comprising a Borrelia burgdorferi polypeptide selected from the group consisting of OspA, OspC, OspF, p39 and a fusion peptide of p41 and VlsE; and
b) detecting a polypeptide-antibody complex formed by a sandwich assay format wherein said Anaplasma phagocytophilum p44 polypeptide, said antibody against a heartworm ( Dirofilaria immitis ) antigen, said Ehrlichia canis gp36 polypeptide, and said Borrelia burgdorferi polypeptide function as capture binders or antibody, and a second labeled binder or antibody is used for generating a detectable signal.
2. The method of claim 1 , wherein the composition in step a) comprises all four of the reagents.
3. The method of claim 1 , wherein the reagent a)(1) is a chicken polyclonal antibody.
4. The method of claim 3 , wherein the chicken polyclonal antibody is produced by immunizing chickens with a canine heartworm antigen.
5. The method of claim 1 , wherein the reagent a)(2) comprises a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:26.
6. The method of claim 5 , wherein the polypeptide comprising the amino acid sequence set forth in SEQ ID NO:26 further comprises a tag sequence.
7. The method of claim 1 , wherein the reagent a)(3) comprises at least two Borrelia burgdorferi polypeptides selected from the group consisting of:
5) an OspA polypeptide,
6) an OspC polypeptide,
7) an OspF polypeptide,
8) a p39 polypeptide, and
9) a fusion peptide of p41 and VlsE,
wherein said antigenic composition does not consist of 5) and 6).
8. The method of claim 7 , wherein the reagent a)(3) comprises at least 3, 4, or all 5 of the Borrelia burgdorferi polypeptides.
9. The method of claim 7 , wherein the OspC polypeptide comprises the amino acid sequence set forth in SEQ ID NO:15.
10. The method of claim 7 , wherein the OspF polypeptide comprises the amino acid sequence set forth in SEQ ID NO:18.
11. The method of claim 7 , wherein the p39 polypeptide comprises the amino acid sequence set forth in SEQ ID NO:21.
12. The method of claim 7 , wherein the fusion peptide of p41 and VlsE comprises the amino acid sequence set forth in SEQ ID NO:24.
13. The method of claim 12 , wherein the fusion peptide of p41 and VlsE further comprises a tag sequence.
14. The method of claim 7 , wherein the method is used for diagnosis, prognosis, stratification, risk assessment, or treatment monitoring of a disease.
15. The method of claim 14 , wherein the disease is selected from the group consisting of a heartworm disease, ehrlichiosis, granulocytic anaplasmosis, and Lyme disease, and
an antibody against a heartworm ( Dirofilaria immitis ) antigen is used for diagnosis, prognosis, stratification, risk assessment, or treatment monitoring of the heartworm disease,
an Ehrlichia canis gp36 polypeptide is used for diagnosis, prognosis, stratification, risk assessment, or treatment monitoring of the ehrlichiosis,
an Anaplasma phagocytophilum p44 polypeptide comprising the amino acid sequence set forth in SEQ ID NO:6 is used for diagnosis, prognosis, stratification, risk assessment, or treatment monitoring of the granulocytic anaplasmosis, and
at least two Borrelia burgdorferi polypeptides selected from the group consisting of an OspA polypeptide, an OspC polypeptide, an OspF polypeptide, a p39 polypeptide and a fusion peptide of p41 and VlsE are used for diagnosis, prognosis, stratification, risk assessment, or treatment monitoring of the Lyme disease.
16. The method of claim 7 , wherein the sample is selected from the group consisting of a serum, a plasma and a blood sample.
17. The method of claim 7 , wherein the sample is a clinical sample.
18. The method of claim 7 , wherein the polypeptide-antibody complex is assessed by a sandwich or competitive assay format, optionally with a binder or antibody.
19. The method of claim 18 , wherein the binder or antibody is attached to a surface and functions as a capture binder or antibody.
20. The method of claim 19 , wherein the capture binder or antibody is attached to the surface directly or indirectly.
21. The method of claim 20 , wherein the capture binder or antibody is attached to the surface via a biotin-avidin (or streptavidin) linking pair.
22. The method of claim 18 , wherein at least one of the binders or antibodies is labeled.
23. The method of claim 7 , wherein the polypeptide-antibody complex is assessed by a sandwich assay format selected from the group consisting of an enzyme-linked immunosorbent assay (ELISA), immunoblotting, immunoprecipitation, radioimmunoassay (MA), immunostaining, chemiluminescence assay, and lateral flow immunoassay.
24. The method of claim 7 , wherein the polypeptide-antibody complex is assessed in a homogeneous assay format.
25. The method of claim 7 , wherein the polypeptide-antibody complex is assessed in a heterogeneous assay format.
26. The method of claim 1 , wherein the Anaplasma phagocytophilum p44 polypeptide comprises an amino acid sequence set forth in SEQ ID NO:7.