Anti-microRNA oligonucleotide molecules
The invention relates to isolated anti-microRNA molecules. In another embodiment, the invention relates to an isolated microRNA molecule. In yet another embodiment, the invention provides a method for inhibiting microRNP activity in a cell.
1. An isolated molecule comprising a maximum of fifty moieties, wherein each moiety comprises a base bonded to a backbone unit, said molecule comprising the microRNA molecule identified in SEQ ID NO: 142 or its corresponding anti-micro RNA molecule identified in SEQ ID NO: 448, wherein the molecule is modified for increased nuclease resistance.
2. The molecule according to claim 1 , wherein at least one of the moieties is a modified ribonucleotide moiety.
3. The molecule according to claim 2 , wherein the modified ribonucleotide is substituted at the 2′ position.
4. The molecule according to claim 3 , wherein the substituent at the 2′ position is a C 1 to C 4 alkyl group.
5. The molecule according to claim 4 , wherein the alkyl group is methyl.
6. The molecule according to claim 4 , wherein the alkyl group is allyl.
7. The molecule according to claim 3 , wherein the substituent at the 2′ position is a C 1 to C 4 alkoxy-C 1 to C 4 alkyl group.
8. The molecule according to claim 7 , wherein the C 1 to C 4 alkoxy-C 1 to C 4 alkyl group is methoxyethyl.
9. The molecule according to claim 1 , wherein at least one of the moieties is a 2′-fluororibonucleotide moiety.
10. The molecule according to claim 2 , wherein the modified ribonucleotide has a methylene bridge between the 2′-oxygen atom and the 4′-carbon atom.
11. The molecule according to claim 1 , wherein the molecule comprises at least one modified moiety on the 5′ end.
12. The molecule according to claim 1 , wherein the molecule comprises at least two modified moieties at the 5′ end.
13. The molecule according to claim 1 , wherein the molecule comprises at least one modified moiety on the 3′ end.
14. The molecule according to claim 1 , wherein the molecule comprises at least two modified moieties at the 3′ end.
15. The molecule according to claim 1 , wherein the molecule comprises at least two modified moieties at the 5′ end and at least two modified moieties at the 3′ end.
16. The molecule according to claim 1 , wherein the molecule comprises a nucleotide cap at the 5′ end, the 3′ end or both.
17. The molecule according to claim 1 , wherein the molecule consists of the microRNA molecule identified in SEQ ID NO: 142.
18. The molecule according to claim 1 , wherein the molecule consists of the anti-micro RNA molecule identified in SEQ ID NO: 448.
19. A vector comprising an isolated nucleic acid molecule according to claim 1 .