Halogenated rhodamine derivatives and applications thereof
Methods for treating an infection in a tissue sample are described. Tissue samples that are harvested from a patient may be infected with either bacteria or a virus. A rhodamine compound is mixed with the infected tissue sample to form a mixture. The mixture is then exposed to radiant energy to inhibit or kill the bacteria. The exposed mixture is then transplanted into the patient. The rhodamine compounds may be 2′-(6-dimethylamino-3-dimethylimino-3H-xanthen-9-yl) 4′, 5′-dichloro-benzoic acid methyl ester hydrochloride.
1. A method for treating a tissue sample from a patient, comprising the steps of:
harvesting the tissue sample from the patient, wherein the tissue sample is infected with bacteria, wherein the bacteria is gram positive;
providing a rhodamine compound, wherein the rhodamine compound is 2′-(6-dimethylamino-3-dimethylimino-3H-xanthen-9-yl) 4′, 5′-dichloro-benzoic acid methyl ester hydrochloride;
mixing the rhodamine compound and the tissue sample to form a mixture,
exposing the mixture to radiant energy to inhibit or kill the bacteria; and
transplanting the exposed mixture into the patient.
2. The method of claim 1 , wherein the bacteria is Staphylococcus epidermitis.
3. The method of claim 1 , wherein the mixture is exposed to a wavelength of 514 nm.
4. The method of claim 1 , wherein the tissue sample is blood.
5. The method of claim 1 , wherein the tissue sample is bone marrow.
6. The method of claim 1 , wherein the mixture is exposed to radiant energy for 180 minutes.
7. The method of claim 1 , further comprising the steps of
centrifuging the mixture;
resuspending the centrifuged mixture; and
incubating the resuspended centrifuged mixture.
8. The method of claim 7 , wherein the resuspended centrifuged mixture is incubated for 90 minutes.
9. The method of claim 7 , wherein the resuspended centrifuged mixture is incubated before exposing the mixture to radiant energy.