Magnetic lysis method and device
View Patent ↗A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
1. A method for lysing cells within a vessel, said method comprising:
placing said vessel on a surface, wherein said vessel comprises one or more cells suspended in a liquid medium, one or more magnetic stirrers, and a plurality of beads; and
rotating a cylinder shaped magnet along an axis, wherein said axis passes the center of said cylinder shaped magnet, is in the proximity of said vessel and is parallel to the surface said vessel resides on so that the vessel is within operational range of a rotating magnetic field produced by said cylinder shaped magnet, and wherein said rotating said cylinder shaped magnet causes said one or more magnetic stirrers in said vessel to collide with said beads with enough force to cause disintegration of said one or more cells, thereby lysing said one or more cells within said vessel.
2. The method of claim 1 , wherein said magnet is a permanent magnet.
3. The method of claim 1 , wherein said rotating said cylinder shaped magnet causes said one or more magnetic stirrers in said vessel to rotate at a speed greater than 1000 rpm.
4. The method of claim 1 , wherein said rotating said cylinder shaped magnet causes said one or more magnetic stirrers in said vessel to rotate for a period of about 1 minute to about 15 minutes.
5. The method of claim 1 , wherein said vessel is in a form selected from the group consisting of vials, test tubes, wells in a microplate, and flow-chambers.
6. The method of claim 1 , wherein said vessel contains two or more types of cells and wherein the rotating step comprises:
rotating said magnet at a first speed that causes lysis of a first type of ceils among said two or more types of cells.
7. The method of claim 6 , wherein the rotating step further comprises:
rotating said magnet at a second speed that causes lysis of a second type of cells among said two or more types of cells, wherein said second type of cells are different from said first type of cells.
8. The method of claim 1 , wherein said plurality of beads comprise glass beads, plastic beads, ceramic beads, metal beads or mixtures thereof.
9. The method of claim 1 , wherein said one or more magnetic stirrer comprises a metal or an alloy.
10. The method of claim 1 , wherein said one or more magnetic stirrer comprises an alloy core coated with a polymer.
11. The method of claim 10 , wherein said polymer comprises a biocompatible polymer.
12. The method of claim 11 , wherein said biocompatible polymer comprises polytetrafluoroethylene (PTFE) or parylene.