Extruded non-replicating probiotic micro-organisms and their health benefits
View Patent ↗The present invention generally relates to the field of probiotic micro-organisms, in particular to the field of non-replicating probiotic micro-organisms. Embodiments of the present invention relate to compositions comprising probiotic micro-organisms that were rendered non-replicating by extrusion. Such compositions may be used to treat or prevent disorders that are related to a compromised immune system.
1. A method for making a composition comprising probiotic micro-organisms rendered non-replicating, the method comprising extruding the probiotic micro-organisms by direct expansion extrusion cooking at a temperature between 110 and 180° C. and at a moisture content below 20 wt %, wherein the probiotic micro-organisms are selected from the group consisting of Bifidobacterium longum NCC 3001, Bifidobacterium longum NCC 2705, Bifidobacterium breve NCC 2950, Bifidobacterium lactis NCC 2818, Lactobacillus johnsonii NCC533, Lactobacillus paracasei NCC 2461, Lactobacillus rhamnosus NCC 4007, Lactobacillus reuteri DSM17938, Lactobacillus reuteri ATCC55730, Streptococcus thermophilus NCC 2019, Streptococcus thermophilus NCC 2059, Lactobacillus casei NCC 4006, Lactobacillus acidophilus NCC 3009, Lactobacillus casei ACA-DC 6002 (NCC 1825), Escherichia coli Nissle, Lactobacillus bulgaricus NCC 15, Lactococcus lactis NCC 2287, and combinations thereof.
2. The method of claim 1 , wherein the probiotic micro-organisms are co-extruded with other parts of the composition.
3. The method of claim 1 comprising adding probiotic micro-organisms to a raw material by dry mixing, extruding the dry mix under water addition, and recovering the extruded material comprising the non replicating probiotic micro-organisms.
4. The method of claim 1 comprising adding a wet mix comprising water and probiotic micro-organisms during an extrusion step to a raw material in powder format, and recovering the extruded material comprising the non replicating probiotic micro-organisms.
5. The method of claim 1 , wherein the direct expansion extrusion cooking occurs at a pressure from 100 to 150 bars.